{"id":31587,"date":"2024-04-15T14:59:00","date_gmt":"2024-04-15T12:59:00","guid":{"rendered":"https:\/\/fhi.nl\/nieuws\/cryogene-opslag-vloeibare-stikstof-of-mechanische-vriezer\/"},"modified":"2024-07-10T12:59:02","modified_gmt":"2024-07-10T10:59:02","slug":"cryogene-opslag-vloeibare-stikstof-of-mechanische-vriezer","status":"publish","type":"news","link":"https:\/\/fhi.nl\/en\/news\/cryogene-opslag-vloeibare-stikstof-of-mechanische-vriezer\/","title":{"rendered":"Cryogenic storage: liquid nitrogen or mechanical freezer?"},"content":{"rendered":"<header id=\"header\" class=\"header header--low header--branch\">\n\n\t\n\t\t\t<div class=\"header__background header__background--graphic\"><\/div>\n\t\n\t<div class=\"container\">\n\t\t<div class=\"header__content\">\n\t\t\t<div class=\"header__first\">\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<h1 class=\"header__title\" >\n\t\t\t\t\tCryogenic storage: liquid nitrogen or mechanical freezer?\t\t\t\t<\/h1>\n\n\t\t\t\t<div class=\"header__dots-line\">\n\t\t\t\t\t<svg width=\"431\" height=\"9\" viewbox=\"0 0 431 9\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M430.799 4.192a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.135 1.135 0 1 1-2.27 0 1.135 1.135 0 0 1 2.27 0Zm-27.27 0a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.39 1.39 0 1 1-2.78 0 1.39 1.39 0 0 1 2.78 0Zm-27.78 0a1.645 1.645 0 1 1-3.29 0 1.645 1.645 0 0 1 3.29 0Zm-28.29 0a1.9 1.9 0 1 1-3.799 0 1.9 1.9 0 0 1 3.799 0Zm-28.799 0a2.154 2.154 0 1 1-4.308 0 2.154 2.154 0 0 1 4.308 0Zm-29.308 0a2.41 2.41 0 1 1-4.819 0 2.41 2.41 0 0 1 4.819 0Zm-29.819 0a2.663 2.663 0 1 1-5.326.001 2.663 2.663 0 0 1 5.326-.001Zm-30.327 0a2.919 2.919 0 1 1-5.837 0 2.919 2.919 0 0 1 5.837 0Zm-30.837 0a3.173 3.173 0 1 1-6.345.001 3.173 3.173 0 0 1 6.345 0Zm-31.346 0a3.428 3.428 0 1 1-6.856 0 3.428 3.428 0 0 1 6.856 0Zm-31.856 0a3.683 3.683 0 1 1-7.365 0 3.683 3.683 0 0 1 7.365 0Zm-32.365 0a3.937 3.937 0 1 1-7.875 0 3.937 3.937 0 0 1 7.875 0Zm-32.874 0a4.192 4.192 0 1 1-8.384 0 4.192 4.192 0 0 1 8.384 0Z\" fill=\"#FFF960\"\/><\/svg>\t\t\t\t<\/div>\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"header__second\">\n\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"header__branch-logos\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/fhi.nl\/app\/uploads\/2024\/02\/Laboratorium-technologie.svg\" class=\"header__branch-logo\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/header>\n\n\t<div class=\"header__meta\">\n\t<div class=\"container\">\n\t\t<div class=\"header__meta__category\">\n\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Branch<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_branches_kennishub=laboratorium-technologie\" class=\"header__meta__detail--branch\">\n\t\t\t\t\t\t\t\tLaboratory Technology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Subject<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=algemene-laboratorium-apparatuur\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tGeneral laboratory equipment\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=laboratorium-technologie\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tLaboratory Technology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/div>\n\n<div class=\"text bg--white\">\n\t<div class=\"container\">\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"text__content text__content--1-col\">\n\t\t\t<p><strong>In short, cryogenic storage is all storage that takes place below -130\u00b0C degrees. It has been proven that at a temperature of -130\u00b0C degrees or colder, all living organisms come to a standstill. Below this temperature, the aging process stops and the cells can be stored indefinitely. The most well-known forms of cryogenic storage are: liquid nitrogen and mechanical freezers. But what are the advantages and disadvantages of these forms and what options are possible? Together with the director of Poly Temp Scientific, Robert van der Kuijlen, Poly Temp discusses these options, so that the customer can make the best choice.<\/strong><em><br \/><\/em><\/p>\r\n<h2>Greater safety margin with liquid nitrogen<\/h2>\r\n<p>Liquid nitrogen (hereinafter LN2) has been used for cryogenic storage for years. In the liquid phase, this form offers a uniform temperature of -196\u00b0C. This means that the temperature remains stable over a longer period of time. Robert adds: &#039;&#039;This temperature of -196\u00b0C gives users confidence in storing their valuable research. If something goes wrong or the LN2 supplier is temporarily unable to deliver, it will take a while before the temperature reaches -130\u00b0C. Without opening the lid, the samples will still remain safe for several days.&#039;&#039;<\/p>\r\n<h3>Store in the liquid or vapor phase?<\/h3>\r\n<p>Samples are often chosen to be stored in the liquid phase due to the certainty of -196\u00b0C, which offers a greater safety margin. Robert: &#039;&#039;Keep in mind that more LN2 is consumed in the liquid phase, because more evaporation takes place when removing a rack. In recent years, we have seen that more and more customers choose to store their samples in the vapor phase.&#039;&#039;<\/p>\r\n<p>In<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S014067369591207X\" target=\"_blank\" rel=\"noopener\"> an article from 1995<\/a> was already written about the possibility of cross-contamination in the liquid phase. &#039;&#039;For that reason, more and more people choose to store in the vapor phase instead of fully immersing the samples in LN2. In the first case, any possibility of cross-contamination is eliminated, but the safety margin is reduced in case the LN2 supplier is temporarily unable to deliver,&#039;&#039; Robert states.<\/p>\r\n<h3>Difference between automatic and manual filling of LN2<\/h3>\r\n<p>In cryogenic storage in LN2 we make a distinction between automatic and manual filling. In automatically filled tanks the LN2 vessel is automatically filled by a system. In the case of manually filled vessels it is up to the user to fill the vessel himself.<\/p>\r\n<p>Manually filled storage vessels have a smaller neck opening. The small neck opening reduces the rate of evaporation of the LN2, as the static evaporation rate is reduced. Automatically filled tanks are more convenient and offer much larger storage capacity. However, due to the larger neck openings and pre-cooling of lines during automatic refilling, they consume more liquid nitrogen.<\/p>\r\n<p><a href=\"https:\/\/polytemp.nl\/uploads\/productbrochures\/cryogeen-opslag\/8.a-cryopreservation-equipment-brochure.pdf\" target=\"_blank\" rel=\"noopener\">Please see the brochure for exact storage quantities per application.<\/a><\/p>\r\n<h3>Ensure safety<\/h3>\r\n<p>It is common knowledge that liquid nitrogen is dangerous to work with. In addition, LN2 has the property that it evaporates, even if the container is sealed airtight. LN2 also extracts oxygen from the air. &#039;&#039;You don&#039;t smell it, you don&#039;t taste it, you don&#039;t see it, but if you are working and the oxygen level is too low, then it becomes dangerous. So it is important to be alert to this,&#039;&#039; says Robert. &#039;&#039;The room in which the LN2 is located must also be equipped with O2 meters. As an additional safety measure, it is recommended that there is always someone outside the room who keeps an eye on the user, in case the alarms in that room do not go off.&#039;&#039;<\/p>\r\n<p>Due to the constant evaporation of LN2, it also remains a continuous process to refill the LN2 vessels. As a result, LN2 must be purchased and budgeted on a consistent basis. Robert: &#039;&#039;Liquid nitrogen certainly provides a larger safety margin in temperature. However, the cost aspect, safety aspect and the fact that liquid nitrogen is not so user-friendly must be taken into account.&#039;&#039;<\/p>\r\n<h2>Mechanical cryogenic freezers as an alternative<\/h2>\r\n<p>Ensuring the safety of laboratory workers is important and some organisations want to do away with liquid nitrogen for that reason. As an alternative, Poly Temp offers a mechanical cryogenic freezer of -150\u00b0C. Firstly, the freezer ensures a nice uniform temperature of -150\u00b0C. This means a safety margin of 20\u00b0C in the event of a power failure, for example. Robert adds: &#039;&#039;With liquid nitrogen, the user can store in the vapour phase, but this is at the expense of storage capacity. With mechanical cooling, this does not apply. The user can then fully utilise the 200 litres that are available for the samples. In addition, it is easier to put material in and take it out of this -150\u00b0C freezer than with an LN2 vessel. The freezer is only one metre high and therefore user-friendly.&#039;&#039;<\/p>\r\n<p>Furthermore, a cryogenic freezer is safer, because no liquid nitrogen is used. Robert: &#039;&#039;After installing the cryogenic freezer(s) in the room, it is important to ensure that the ambient temperature is correct. Although this -150\u00b0C freezer is energy-efficient, heat emission must be taken into account. This makes it important that the room is well-conditioned. This will affect power consumption. Finally, the freezer produces a noise level of around 55 dB.&#039;&#039;<\/p>\r\n<p><a href=\"https:\/\/polytemp.nl\/producten\/koude-opslag\/cryopreservation-systems\/mechanische-cryogeenvriezer\" target=\"_blank\" rel=\"noopener\">Check out the -150\u00b0C cryogenic freezer with a good storage capacity from Poly Temp&#039;s own brand Labselect. <\/a><\/p>\r\n<h3>Precautions for Cryogenic Freezers<\/h3>\r\n<p>For example, if the freezer fails due to a power failure, the freezer will slowly start to warm up. Robert: &#039;&#039;If the freezer fails, for whatever reason, I always advise to be prepared for this. First of all, I advise to connect the freezer to an emergency generator of the building. Secondly, to connect an LN2 backup system to the freezer. In the event of a failure, LN2, in vapor form, can be injected into the freezer, so that the material remains below the critical temperature of -130\u00b0C. For this, an LN2 filling tank must be ready with LN2.&#039;&#039;<\/p>\r\n<blockquote>\r\n<p><em>Calculation of the Total Cost of Ownership<\/em><\/p>\r\n<p>Robert concludes: &#039;&#039;We offer both options at Poly Temp so whether you need an LN2 storage tank or a mechanical freezer, it is entirely up to the customer. Both systems offer cryogenic temperatures for storing valuable samples.<\/p>\r\n<p>We do recommend that you calculate the TCO over the longer term and include this in the final decision.<\/p>\r\n<p>In the calculation, the comparison is made between these forms of cryogenic storage. Liquid nitrogen requires a specially equipped space and delivery of nitrogen. In comparison with our cryogenic freezer with electricity costs and climate control systems. Of course, it is possible to submit the issue to us and we are happy to do the calculation together with our customer.&#039;&#039;<\/p>\r\n<\/blockquote>\r\n<p><a href=\"https:\/\/polytemp.nl\/producten\/koude-opslag\/cryopreservation-systems\" target=\"_blank\" rel=\"noopener\">View the cryogenic storage range at polytemp.nl.<\/a><\/p>\t\t<\/div>\n\t<\/div>\n<\/div>\r\n\t<div class=\"articles bg--offwhite automatic\">\r\n\t\t<div class=\"container\">\r\n\t\t\t<div class=\"articles__header\">\r\n\t\t\t\t\t\t\t\t\t<div class='heading-wrapper'><svg width=\"13\" height=\"13\" viewbox=\"0 0 13 13\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><circle cx=\"6.394\" cy=\"6.5\" r=\"6.394\" fill=\"#000\"\/><\/svg><h2>Related articles<\/h2><\/div>\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"\/en\/profiel\/poly-temp-scientific\/\" class=\"button button--outline\">view profile<\/a>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t<div class=\"post-grid post-grid--no-padding\">\r\n\t\t\t\t\n<a 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96.958 48.479C96.958 21.7048 75.2532 0 48.479 0C21.7048 0 0 21.7048 0 48.479C0 75.2532 21.7048 96.958 48.479 96.958ZM50.8409 13.4297C50.8409 12.3702 49.982 11.5112 48.9224 11.5112C47.8629 11.5112 47.0039 12.3702 47.0039 13.4297V24.9407C47.0039 26.0003 47.8629 26.8592 48.9224 26.8592C49.982 26.8592 50.8409 26.0003 50.8409 24.9407V13.4297ZM67.5169 16.0418C68.4345 16.5716 68.7489 17.7449 68.2192 18.6625L62.8261 28.0036C62.2963 28.9212 61.123 29.2356 60.2054 28.7058C59.2878 28.176 58.9734 27.0027 59.5031 26.0851L64.8962 16.744C65.426 15.8264 66.5993 15.512 67.5169 16.0418ZM29.7398 18.6625C29.21 17.7449 29.5244 16.5716 30.442 16.0418C31.3596 15.512 32.533 15.8264 33.0628 16.744L38.4558 26.0851C38.9856 27.0027 38.6712 28.176 37.7536 28.7058C36.836 29.2356 35.6627 28.9212 35.1329 28.0036L29.7398 18.6625ZM81.7542 29.5272C82.284 30.4448 81.9696 31.6181 81.0519 32.1479L71.7109 37.541C70.7933 38.0708 69.6199 37.7564 69.0901 36.8388C68.5604 35.9212 68.8748 34.7478 69.7924 34.2181L79.1335 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2021\t\t\t<\/div>\n\t\t\t<\/div>\n<\/a>\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":31588,"template":"","branches":[14],"events":[50,360,8],"secretariat":[],"categories":[26,59],"themes_tax":[],"content_types":[514],"class_list":["post-31587","news","type-news","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Cryogene opslag: vloeibare stikstof of mechanische vriezer?<\/title>\n<meta name=\"description\" content=\"Wat zijn de voor- en nadelen van deze vormen en welke opties zijn daarbij mogelijk? 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