{"id":31587,"date":"2024-04-15T14:59:00","date_gmt":"2024-04-15T12:59:00","guid":{"rendered":"https:\/\/fhi.nl\/nieuws\/cryogene-opslag-vloeibare-stikstof-of-mechanische-vriezer\/"},"modified":"2024-07-10T12:59:02","modified_gmt":"2024-07-10T10:59:02","slug":"cryogene-opslag-vloeibare-stikstof-of-mechanische-vriezer","status":"publish","type":"news","link":"https:\/\/fhi.nl\/en\/news\/cryogene-opslag-vloeibare-stikstof-of-mechanische-vriezer\/","title":{"rendered":"Cryogenic storage: liquid nitrogen or mechanical freezer?"},"content":{"rendered":"<header id=\"header\" class=\"header header--low header--branch\">\n\n\t\n\t\t\t<div class=\"header__background header__background--graphic\"><\/div>\n\t\n\t<div class=\"container\">\n\t\t<div class=\"header__content\">\n\t\t\t<div class=\"header__first\">\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<h1 class=\"header__title\" >\n\t\t\t\t\tCryogenic storage: liquid nitrogen or mechanical freezer?\t\t\t\t<\/h1>\n\n\t\t\t\t<div class=\"header__dots-line\">\n\t\t\t\t\t<svg width=\"431\" height=\"9\" viewbox=\"0 0 431 9\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M430.799 4.192a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.135 1.135 0 1 1-2.27 0 1.135 1.135 0 0 1 2.27 0Zm-27.27 0a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.39 1.39 0 1 1-2.78 0 1.39 1.39 0 0 1 2.78 0Zm-27.78 0a1.645 1.645 0 1 1-3.29 0 1.645 1.645 0 0 1 3.29 0Zm-28.29 0a1.9 1.9 0 1 1-3.799 0 1.9 1.9 0 0 1 3.799 0Zm-28.799 0a2.154 2.154 0 1 1-4.308 0 2.154 2.154 0 0 1 4.308 0Zm-29.308 0a2.41 2.41 0 1 1-4.819 0 2.41 2.41 0 0 1 4.819 0Zm-29.819 0a2.663 2.663 0 1 1-5.326.001 2.663 2.663 0 0 1 5.326-.001Zm-30.327 0a2.919 2.919 0 1 1-5.837 0 2.919 2.919 0 0 1 5.837 0Zm-30.837 0a3.173 3.173 0 1 1-6.345.001 3.173 3.173 0 0 1 6.345 0Zm-31.346 0a3.428 3.428 0 1 1-6.856 0 3.428 3.428 0 0 1 6.856 0Zm-31.856 0a3.683 3.683 0 1 1-7.365 0 3.683 3.683 0 0 1 7.365 0Zm-32.365 0a3.937 3.937 0 1 1-7.875 0 3.937 3.937 0 0 1 7.875 0Zm-32.874 0a4.192 4.192 0 1 1-8.384 0 4.192 4.192 0 0 1 8.384 0Z\" fill=\"#FFF960\"\/><\/svg>\t\t\t\t<\/div>\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"header__second\">\n\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"header__branch-logos\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/fhi.nl\/app\/uploads\/2024\/02\/Laboratorium-technologie.svg\" class=\"header__branch-logo\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/header>\n\n\t<div class=\"header__meta\">\n\t<div class=\"container\">\n\t\t<div class=\"header__meta__category\">\n\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Branch<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_branches_kennishub=laboratorium-technologie\" class=\"header__meta__detail--branch\">\n\t\t\t\t\t\t\t\tLaboratory Technology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Subject<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=algemene-laboratorium-apparatuur\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tGeneral laboratory equipment\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=laboratorium-technologie\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tLaboratory Technology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/div>\n\n<div class=\"text bg--white\">\n\t<div class=\"container\">\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"text__content text__content--1-col\">\n\t\t\t<p><strong>In short, cryogenic storage is all storage that takes place below -130\u00b0C degrees. It has been proven that at a temperature of -130\u00b0C degrees or colder, all living organisms come to a standstill. Below this temperature, the aging process stops and the cells can be stored indefinitely. The most well-known forms of cryogenic storage are: liquid nitrogen and mechanical freezers. But what are the advantages and disadvantages of these forms and what options are possible? Together with the director of Poly Temp Scientific, Robert van der Kuijlen, Poly Temp discusses these options, so that the customer can make the best choice.<\/strong><em><br \/><\/em><\/p>\r\n<h2>Greater safety margin with liquid nitrogen<\/h2>\r\n<p>Liquid nitrogen (hereinafter LN2) has been used for cryogenic storage for years. In the liquid phase, this form offers a uniform temperature of -196\u00b0C. This means that the temperature remains stable over a longer period of time. Robert adds: &#039;&#039;This temperature of -196\u00b0C gives users confidence in storing their valuable research. If something goes wrong or the LN2 supplier is temporarily unable to deliver, it will take a while before the temperature reaches -130\u00b0C. Without opening the lid, the samples will still remain safe for several days.&#039;&#039;<\/p>\r\n<h3>Store in the liquid or vapor phase?<\/h3>\r\n<p>Samples are often chosen to be stored in the liquid phase due to the certainty of -196\u00b0C, which offers a greater safety margin. Robert: &#039;&#039;Keep in mind that more LN2 is consumed in the liquid phase, because more evaporation takes place when removing a rack. In recent years, we have seen that more and more customers choose to store their samples in the vapor phase.&#039;&#039;<\/p>\r\n<p>In<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S014067369591207X\" target=\"_blank\" rel=\"noopener\"> an article from 1995<\/a> was already written about the possibility of cross-contamination in the liquid phase. &#039;&#039;For that reason, more and more people choose to store in the vapor phase instead of fully immersing the samples in LN2. In the first case, any possibility of cross-contamination is eliminated, but the safety margin is reduced in case the LN2 supplier is temporarily unable to deliver,&#039;&#039; Robert states.<\/p>\r\n<h3>Difference between automatic and manual filling of LN2<\/h3>\r\n<p>In cryogenic storage in LN2 we make a distinction between automatic and manual filling. In automatically filled tanks the LN2 vessel is automatically filled by a system. In the case of manually filled vessels it is up to the user to fill the vessel himself.<\/p>\r\n<p>Manually filled storage vessels have a smaller neck opening. The small neck opening reduces the rate of evaporation of the LN2, as the static evaporation rate is reduced. Automatically filled tanks are more convenient and offer much larger storage capacity. However, due to the larger neck openings and pre-cooling of lines during automatic refilling, they consume more liquid nitrogen.<\/p>\r\n<p><a href=\"https:\/\/polytemp.nl\/uploads\/productbrochures\/cryogeen-opslag\/8.a-cryopreservation-equipment-brochure.pdf\" target=\"_blank\" rel=\"noopener\">Please see the brochure for exact storage quantities per application.<\/a><\/p>\r\n<h3>Ensure safety<\/h3>\r\n<p>It is common knowledge that liquid nitrogen is dangerous to work with. In addition, LN2 has the property that it evaporates, even if the container is sealed airtight. LN2 also extracts oxygen from the air. &#039;&#039;You don&#039;t smell it, you don&#039;t taste it, you don&#039;t see it, but if you are working and the oxygen level is too low, then it becomes dangerous. So it is important to be alert to this,&#039;&#039; says Robert. &#039;&#039;The room in which the LN2 is located must also be equipped with O2 meters. As an additional safety measure, it is recommended that there is always someone outside the room who keeps an eye on the user, in case the alarms in that room do not go off.&#039;&#039;<\/p>\r\n<p>Due to the constant evaporation of LN2, it also remains a continuous process to refill the LN2 vessels. As a result, LN2 must be purchased and budgeted on a consistent basis. Robert: &#039;&#039;Liquid nitrogen certainly provides a larger safety margin in temperature. However, the cost aspect, safety aspect and the fact that liquid nitrogen is not so user-friendly must be taken into account.&#039;&#039;<\/p>\r\n<h2>Mechanical cryogenic freezers as an alternative<\/h2>\r\n<p>Ensuring the safety of laboratory workers is important and some organisations want to do away with liquid nitrogen for that reason. As an alternative, Poly Temp offers a mechanical cryogenic freezer of -150\u00b0C. Firstly, the freezer ensures a nice uniform temperature of -150\u00b0C. This means a safety margin of 20\u00b0C in the event of a power failure, for example. Robert adds: &#039;&#039;With liquid nitrogen, the user can store in the vapour phase, but this is at the expense of storage capacity. With mechanical cooling, this does not apply. The user can then fully utilise the 200 litres that are available for the samples. In addition, it is easier to put material in and take it out of this -150\u00b0C freezer than with an LN2 vessel. The freezer is only one metre high and therefore user-friendly.&#039;&#039;<\/p>\r\n<p>Furthermore, a cryogenic freezer is safer, because no liquid nitrogen is used. Robert: &#039;&#039;After installing the cryogenic freezer(s) in the room, it is important to ensure that the ambient temperature is correct. Although this -150\u00b0C freezer is energy-efficient, heat emission must be taken into account. This makes it important that the room is well-conditioned. This will affect power consumption. Finally, the freezer produces a noise level of around 55 dB.&#039;&#039;<\/p>\r\n<p><a href=\"https:\/\/polytemp.nl\/producten\/koude-opslag\/cryopreservation-systems\/mechanische-cryogeenvriezer\" target=\"_blank\" rel=\"noopener\">Check out the -150\u00b0C cryogenic freezer with a good storage capacity from Poly Temp&#039;s own brand Labselect. <\/a><\/p>\r\n<h3>Precautions for Cryogenic Freezers<\/h3>\r\n<p>For example, if the freezer fails due to a power failure, the freezer will slowly start to warm up. Robert: &#039;&#039;If the freezer fails, for whatever reason, I always advise to be prepared for this. First of all, I advise to connect the freezer to an emergency generator of the building. Secondly, to connect an LN2 backup system to the freezer. In the event of a failure, LN2, in vapor form, can be injected into the freezer, so that the material remains below the critical temperature of -130\u00b0C. For this, an LN2 filling tank must be ready with LN2.&#039;&#039;<\/p>\r\n<blockquote>\r\n<p><em>Calculation of the Total Cost of Ownership<\/em><\/p>\r\n<p>Robert concludes: &#039;&#039;We offer both options at Poly Temp so whether you need an LN2 storage tank or a mechanical freezer, it is entirely up to the customer. Both systems offer cryogenic temperatures for storing valuable samples.<\/p>\r\n<p>We do recommend that you calculate the TCO over the longer term and include this in the final decision.<\/p>\r\n<p>In the calculation, the comparison is made between these forms of cryogenic storage. Liquid nitrogen requires a specially equipped space and delivery of nitrogen. In comparison with our cryogenic freezer with electricity costs and climate control systems. Of course, it is possible to submit the issue to us and we are happy to do the calculation together with our customer.&#039;&#039;<\/p>\r\n<\/blockquote>\r\n<p><a href=\"https:\/\/polytemp.nl\/producten\/koude-opslag\/cryopreservation-systems\" target=\"_blank\" rel=\"noopener\">View the cryogenic storage range at polytemp.nl.<\/a><\/p>\t\t<\/div>\n\t<\/div>\n<\/div>\r\n\t<div class=\"articles bg--offwhite automatic\">\r\n\t\t<div class=\"container\">\r\n\t\t\t<div class=\"articles__header\">\r\n\t\t\t\t\t\t\t\t\t<div class='heading-wrapper'><svg width=\"13\" height=\"13\" viewbox=\"0 0 13 13\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><circle cx=\"6.394\" cy=\"6.5\" r=\"6.394\" fill=\"#000\"\/><\/svg><h2>Related articles<\/h2><\/div>\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"\/en\/profiel\/poly-temp-scientific\/\" class=\"button button--outline\">view profile<\/a>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t<div class=\"post-grid post-grid--no-padding\">\r\n\t\t\t\t\n<a 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86.0205L62.8152 61.8148Z\" fill=\"#FFDA56\"\/>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>PreeKem P3 automates microwave digestion<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Laboratory Technology<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Quality<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Laboratory automation<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Analysis<\/span>\n\t\t\t\t\t<\/div>\n\t<div class=\"single-item__articles-author-date-wrapper\">\n\t\t\t\t\t<div class=\"single-item__articles-author\">\n\t\t\t\ta1-envirosciences 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15.3755 24.5619 14.3215 23.8956 13.2138C23.0294 11.7738 22.5586 9.91326 22.5586 7.96501C22.5586 6.01675 23.0294 4.15622 23.8956 2.71619C24.7383 1.31517 26.1797 0 28.1466 0H55.0564C57.0232 0 58.4646 1.31517 59.3073 2.71619C60.1735 4.15622 60.6444 6.01675 60.6444 7.96501C60.6444 9.91326 60.1735 11.7738 59.3073 13.2138C58.4646 14.6148 57.0232 15.93 55.0564 15.93H54.796V40.3243L80.5327 84.0062L80.5391 84.0172L80.5503 84.0368C84.0711 90.2609 79.5954 97.9997 72.4362 97.9997H9.34052C2.18152 97.9997 -2.29415 90.2608 1.22665 84.0368C1.23243 84.0265 1.23831 84.0163 1.24428 84.0062L26.981 40.3243V15.7683ZM30.981 15.93V35.792H39.161C40.2655 35.792 41.161 36.6874 41.161 37.792C41.161 38.8966 40.2655 39.792 39.161 39.792H30.981V40.8697C30.981 41.2268 30.8854 41.5773 30.7041 41.8849L21.3184 57.8149H60.4585L51.0728 41.8849C50.8916 41.5773 50.796 41.2268 50.796 40.8697V15.93H30.981ZM62.8152 61.8148C62.8065 61.8149 62.7978 61.8149 62.7891 61.8149H19.4727C19.3082 61.8149 19.1484 61.7951 18.9954 61.7577L4.70016 86.0205C2.69212 89.5942 5.27194 93.9997 9.34052 93.9997H72.4362C76.5051 93.9997 79.0848 89.5941 77.0768 86.0205L62.8152 61.8148Z\" fill=\"#FFDA56\"\/>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>100 years of Beun-De Ronde<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Laboratory Technology<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">World of Industry, Technology &amp; Science<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Service, maintenance and repair<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Service, maintenance and repair (services)<\/span>\n\t\t\t\t\t<\/div>\n\t<div class=\"single-item__articles-author-date-wrapper\">\n\t\t\t\t\t<div class=\"single-item__articles-author\">\n\t\t\t\tBeun-De Ronde\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"single-item__articles-date\">\n\t\t\t\tAugust 19, 2021\t\t\t<\/div>\n\t\t\t<\/div>\n<\/a>\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"featured_media":31588,"template":"","branches":[14],"events":[50,360,8],"secretariat":[],"categories":[26,59],"themes_tax":[],"content_types":[514],"class_list":["post-31587","news","type-news","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Cryogene opslag: vloeibare stikstof of mechanische vriezer?<\/title>\n<meta name=\"description\" content=\"Wat zijn de voor- en nadelen van deze vormen en welke opties zijn daarbij mogelijk? 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