{"id":31716,"date":"2024-03-20T11:58:00","date_gmt":"2024-03-20T10:58:00","guid":{"rendered":"https:\/\/fhi.nl\/nieuws\/bioluminescentie-maakt-snelle-dna-detectie-in-infectiediagnostiek-mogelijk\/"},"modified":"2025-11-17T16:33:39","modified_gmt":"2025-11-17T15:33:39","slug":"bioluminescentie-maakt-snelle-dna-detectie-in-infectiediagnostiek-mogelijk","status":"publish","type":"news","link":"https:\/\/fhi.nl\/en\/news\/bioluminescentie-maakt-snelle-dna-detectie-in-infectiediagnostiek-mogelijk\/","title":{"rendered":"Bioluminescence enables rapid DNA detection in infection diagnostics"},"content":{"rendered":"<header id=\"header\" class=\"header header--low header--branch\">\n\n\t\n\t\t\t<div class=\"header__background header__background--graphic\"><\/div>\n\t\n\t<div class=\"container\">\n\t\t<div class=\"header__content\">\n\t\t\t<div class=\"header__first\">\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<h6 class=\"header__subtitle\">By: Eline te Velde<\/h6>\n\t\t\t\t\n\t\t\t\t<h1 class=\"header__title\" >\n\t\t\t\t\tBioluminescence enables rapid DNA detection in infection diagnostics\t\t\t\t<\/h1>\n\n\t\t\t\t<div class=\"header__dots-line\">\n\t\t\t\t\t<svg width=\"431\" height=\"9\" viewbox=\"0 0 431 9\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M430.799 4.192a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.135 1.135 0 1 1-2.27 0 1.135 1.135 0 0 1 2.27 0Zm-27.27 0a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.39 1.39 0 1 1-2.78 0 1.39 1.39 0 0 1 2.78 0Zm-27.78 0a1.645 1.645 0 1 1-3.29 0 1.645 1.645 0 0 1 3.29 0Zm-28.29 0a1.9 1.9 0 1 1-3.799 0 1.9 1.9 0 0 1 3.799 0Zm-28.799 0a2.154 2.154 0 1 1-4.308 0 2.154 2.154 0 0 1 4.308 0Zm-29.308 0a2.41 2.41 0 1 1-4.819 0 2.41 2.41 0 0 1 4.819 0Zm-29.819 0a2.663 2.663 0 1 1-5.326.001 2.663 2.663 0 0 1 5.326-.001Zm-30.327 0a2.919 2.919 0 1 1-5.837 0 2.919 2.919 0 0 1 5.837 0Zm-30.837 0a3.173 3.173 0 1 1-6.345.001 3.173 3.173 0 0 1 6.345 0Zm-31.346 0a3.428 3.428 0 1 1-6.856 0 3.428 3.428 0 0 1 6.856 0Zm-31.856 0a3.683 3.683 0 1 1-7.365 0 3.683 3.683 0 0 1 7.365 0Zm-32.365 0a3.937 3.937 0 1 1-7.875 0 3.937 3.937 0 0 1 7.875 0Zm-32.874 0a4.192 4.192 0 1 1-8.384 0 4.192 4.192 0 0 1 8.384 0Z\" fill=\"#FFF960\"\/><\/svg>\t\t\t\t<\/div>\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"header__second\">\n\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"header__branch-logos\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/fhi.nl\/app\/uploads\/2024\/02\/Laboratorium-technologie.svg\" class=\"header__branch-logo\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/header>\n\n\t<div class=\"header__meta\">\n\t<div class=\"container\">\n\t\t<div class=\"header__meta__category\">\n\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Branch<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_branches_kennishub=laboratorium-technologie\" class=\"header__meta__detail--branch\">\n\t\t\t\t\t\t\t\tLaboratory Technology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/div>\n\n\n\n<div class=\"text bg--offwhite\">\n\t<div class=\"container\">\n\t\t\t\t\t\t<div class=\"text__content text__content--1-col\">\n\t\t\t<p><strong>Imagine: a test with the accuracy of PCR, but faster and simpler. Researchers from Eindhoven University of Technology have developed a new method for the detection of DNA\/RNA. PhD student Yosta de Stigter talks about the operation and application of this groundbreaking technology. <\/strong><\/p>\n<p>Polymerase Chain Reaction (PCR) is a reliable and widely used method for detecting bacteria and viruses. Through several cycles of heating and cooling, the DNA multiplies and tiny amounts of viral DNA are made detectable. PCR requires specialist equipment and must be carried out in the laboratory. In practice, this means that the patient has to wait one or more days for a test result. TU Eindhoven and Fontys University of Applied Sciences are working together on an alternative in which the GP makes the diagnosis himself and the patient receives the results within half an hour.<\/p>\n<p>Yosta is investigating the possibility of using this new method to test for STDs. \u201cThere is a taboo on the subject. Many people find it difficult to go to the GGD or the GP and ask for a test. It sometimes takes two days until you receive the test results, and during that time you cannot yet start a treatment plan. We hope to accelerate this process and lower the barrier to testing.\u201d<\/p>\n<p>But the researchers mainly see opportunities in countries where central laboratories are not always available. \u201cInfectious diseases, such as malaria, are still a major problem. Simple and cheap tests can really make a big difference.\u201d<\/p>\n<h4>LUNAS<\/h4>\n<p>Researchers from Eindhoven University of Technology have developed a Luminescent Nucleic Acid Sensor (LUNAS). LUNAS uses so-called <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acscentsci.2c01467\">luciferase enzymes<\/a>. These enzymes cause bioluminescence and thus emit light, such as in fireflies and glowworms. Yosta explains how it works: \u201cThe sensor detects the DNA of viruses or bacteria in a sample. To find the right DNA we use the D-Cas9 protein.\u201d These proteins are designed to detect and bind to a very specific piece of DNA, for example from a virus.<\/p>\n<p>\u201cThe luciferase enzymes help us detect the DNA,\u201d Yosta continues. \u201cWe split this into two pieces and attach it to two D-Cas9 proteins. The proteins look for the target DNA and bind to it. When this happens, the two pieces of luciferase enzyme come together and emit light. If the target DNA is not present, the D-Cas-9 proteins do not find each other and it remains dark.\u201d The scam is then made visible with a digital camera.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<td><em>The Cas9 protein is known from the CRISPR-Cas technique. This technique allows scientists to edit the DNA of organisms very precisely by cutting, replacing or adding pieces of genetic material. LUNAS uses the D-Cas9 protein. &#039;D&#039; stands for dead. The protein therefore no longer cuts, but only binds to the piece of DNA it is looking for<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Amplification<\/h4>\n<p>Finding a specific piece of DNA in a microliter sample is highly specialized work. \u201cYou can compare it to looking for one fish in Lake Victoria in Africa,\u201d says Yosta. \u201cPCR is so sensitive because it detects a tiny amount of DNA in a sample by amplifying it. The power of DNA is that we can amplify it, which is why DNA tests are the current standard in diagnostics.\u201d<\/p>\n<p>Yosta explains that the LUNAS method itself is not sensitive enough to detect clinically relevant samples, and is therefore combined with an amplification technique. The difference with PCR is that the researchers use an amplification technique that works at a single constant temperature. \u201cWe therefore only need two components, something that heats and a camera that can capture the light. This makes the test a lot easier and faster than a PCR test. The combination of speed and a simplified reading method means that in theory you can do this type of test at your GP.\u201d<\/p>\n<blockquote><p><em>\u201cThe combination of speed and a simplified reading method means that in theory you can do this type of test at your GP.\u201d<\/em><\/p><\/blockquote>\n<h4>From theory to practice<\/h4>\n<p>In theory, the LUNAS test can easily be performed at the GP, but to put this into practice, Yosta hopes to integrate the test into a microfluidics device. \u201cWe now work with a large box with a camera in it, but that is of course not a setup that the GP can work with. Moreover, it still requires a relatively large amount of laboratory work. We want to make the test more user-friendly.\u201d<\/p>\n<p>According to Yosta, it will certainly take a few more years before the first general practitioners can start using the LUNAS test. \u201cI don&#039;t know whether all general practitioners will be using exactly this test in ten years&#039; time, but I dare say that test results will become faster and diagnostics will change in the coming years.\u201d<\/p>\n\t\t<\/div>\n\t<\/div>\n<\/div>\n\n\r\n\t<div class=\"articles bg--dark-blue automatic\">\r\n\t\t<div class=\"container\">\r\n\t\t\t<div class=\"articles__header\">\r\n\t\t\t\t\t\t\t\t\t<div class='heading-wrapper'><svg width=\"13\" height=\"13\" viewbox=\"0 0 13 13\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><circle cx=\"6.394\" cy=\"6.5\" r=\"6.394\" fill=\"#000\"\/><\/svg><h2>Read more<\/h2><\/div>\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"\/en\/profiel\/fhi-federatie-van-technologie-branches\/\" class=\"button button--outline\">view profile<\/a>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t<div class=\"post-grid post-grid--no-padding\">\r\n\t\t\t\t\n<a class=\"single-item single-item__articles\" href=\"https:\/\/fhi.nl\/en\/news\/ymif-engineering-verzorgt-de-besturing-van-de-nieuwe-energy-hub-in-tholen\/\" data-id=\"31217\">\n\t<div class=\"single-item__articles-icon\">\n\t\t<svg width=\"103\" height=\"103\" viewbox=\"0 0 103 103\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n<path fill-rule=\"evenodd\" clip-rule=\"evenodd\" d=\"M49.5 98.9587V82.3076C33.3844 81.2758 20.6328 67.878 20.6328 51.5019C20.6328 35.1258 33.3844 21.728 49.5 20.6962V4.04134C24.1941 5.08952 4 25.9365 4 51.5C4 77.0635 24.1941 97.9105 49.5 98.9587ZM53.5 78.2983V61.8096H76.3238C72.5238 70.9498 63.8222 77.5397 53.5 78.2983ZM49.5 78.298V61.8096H26.681C30.4804 70.9484 39.1799 77.5377 49.5 78.298ZM47.0728 57.8096H25.3773C24.8906 55.7865 24.6328 53.6743 24.6328 51.5019C24.6328 37.3358 35.5955 25.7301 49.5 24.7058V37.013H34.0466C33.2709 37.013 32.5653 37.4615 32.2359 38.1637C31.9065 38.8659 32.0128 39.6952 32.5086 40.2916L47.0728 57.8096ZM55.9249 57.8096H77.6274C78.1141 55.7865 78.3719 53.6743 78.3719 51.5019C78.3719 37.3342 67.4068 25.7275 53.5 24.7055V37.013H68.9511C69.7268 37.013 70.4324 37.4615 70.7618 38.1637C71.0912 38.8659 70.9849 39.6952 70.4891 40.2916L55.9249 57.8096ZM53.5 82.3079V98.9587C78.8059 97.9105 99 77.0635 99 51.5C99 25.9365 78.8059 5.08952 53.5 4.04134V20.6959C69.6178 21.7254 82.3719 35.1242 82.3719 51.5019C82.3719 67.8796 69.6178 81.2784 53.5 82.3079ZM0 51.5C0 23.7271 21.9843 1.08883 49.5 0.0381213V0H51.5H53.5V0.0381213C81.0157 1.08883 103 23.7271 103 51.5C103 79.9427 79.9427 103 51.5 103C23.0573 103 0 79.9427 0 51.5ZM38.3102 41.013H64.6875L51.4989 56.8765L38.3102 41.013Z\" fill=\"#2DD881\"\/>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>Ymif Engineering is responsible for the control of the new energy hub in Tholen<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Industrial Electronics<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Development club<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Members content<\/span>\n\t\t\t\t\t<\/div>\n\t<div class=\"single-item__articles-author-date-wrapper\">\n\t\t\t\t\t<div class=\"single-item__articles-author\">\n\t\t\t\tYmif Engineering\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"single-item__articles-date\">\n\t\t\t\tJune 18, 2024\t\t\t<\/div>\n\t\t\t<\/div>\n<\/a>\n\n<a class=\"single-item single-item__articles\" href=\"https:\/\/fhi.nl\/en\/news\/ni-announces-industry-leading-pxi-vst\/\" data-id=\"34109\">\n\t<div class=\"single-item__articles-icon\">\n\t\t<svg width=\"103\" 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82.3079V98.9587C78.8059 97.9105 99 77.0635 99 51.5C99 25.9365 78.8059 5.08952 53.5 4.04134V20.6959C69.6178 21.7254 82.3719 35.1242 82.3719 51.5019C82.3719 67.8796 69.6178 81.2784 53.5 82.3079ZM0 51.5C0 23.7271 21.9843 1.08883 49.5 0.0381213V0H51.5H53.5V0.0381213C81.0157 1.08883 103 23.7271 103 51.5C103 79.9427 79.9427 103 51.5 103C23.0573 103 0 79.9427 0 51.5ZM38.3102 41.013H64.6875L51.4989 56.8765L38.3102 41.013Z\" fill=\"#2DD881\"\/>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>NI ANNOUNCES INDUSTRY LEADING PXI VST<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Industrial Electronics<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Design Automation &amp; Embedded Systems Event<\/span>\n\t\t\t\t\t\t\t\t<span 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