{"id":31716,"date":"2024-03-20T11:58:00","date_gmt":"2024-03-20T10:58:00","guid":{"rendered":"https:\/\/fhi.nl\/nieuws\/bioluminescentie-maakt-snelle-dna-detectie-in-infectiediagnostiek-mogelijk\/"},"modified":"2025-11-17T16:33:39","modified_gmt":"2025-11-17T15:33:39","slug":"bioluminescentie-maakt-snelle-dna-detectie-in-infectiediagnostiek-mogelijk","status":"publish","type":"news","link":"https:\/\/fhi.nl\/en\/news\/bioluminescentie-maakt-snelle-dna-detectie-in-infectiediagnostiek-mogelijk\/","title":{"rendered":"Bioluminescence enables rapid DNA detection in infection diagnostics"},"content":{"rendered":"<header id=\"header\" class=\"header header--low header--branch\">\n\n\t\n\t\t\t<div class=\"header__background header__background--graphic\"><\/div>\n\t\n\t<div class=\"container\">\n\t\t<div class=\"header__content\">\n\t\t\t<div class=\"header__first\">\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<h6 class=\"header__subtitle\">By: Eline te Velde<\/h6>\n\t\t\t\t\n\t\t\t\t<h1 class=\"header__title\" >\n\t\t\t\t\tBioluminescence enables rapid DNA detection in infection diagnostics\t\t\t\t<\/h1>\n\n\t\t\t\t<div class=\"header__dots-line\">\n\t\t\t\t\t<svg width=\"431\" height=\"9\" viewbox=\"0 0 431 9\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M430.799 4.192a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.135 1.135 0 1 1-2.27 0 1.135 1.135 0 0 1 2.27 0Zm-27.27 0a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.39 1.39 0 1 1-2.78 0 1.39 1.39 0 0 1 2.78 0Zm-27.78 0a1.645 1.645 0 1 1-3.29 0 1.645 1.645 0 0 1 3.29 0Zm-28.29 0a1.9 1.9 0 1 1-3.799 0 1.9 1.9 0 0 1 3.799 0Zm-28.799 0a2.154 2.154 0 1 1-4.308 0 2.154 2.154 0 0 1 4.308 0Zm-29.308 0a2.41 2.41 0 1 1-4.819 0 2.41 2.41 0 0 1 4.819 0Zm-29.819 0a2.663 2.663 0 1 1-5.326.001 2.663 2.663 0 0 1 5.326-.001Zm-30.327 0a2.919 2.919 0 1 1-5.837 0 2.919 2.919 0 0 1 5.837 0Zm-30.837 0a3.173 3.173 0 1 1-6.345.001 3.173 3.173 0 0 1 6.345 0Zm-31.346 0a3.428 3.428 0 1 1-6.856 0 3.428 3.428 0 0 1 6.856 0Zm-31.856 0a3.683 3.683 0 1 1-7.365 0 3.683 3.683 0 0 1 7.365 0Zm-32.365 0a3.937 3.937 0 1 1-7.875 0 3.937 3.937 0 0 1 7.875 0Zm-32.874 0a4.192 4.192 0 1 1-8.384 0 4.192 4.192 0 0 1 8.384 0Z\" fill=\"#FFF960\"\/><\/svg>\t\t\t\t<\/div>\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"header__second\">\n\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"header__branch-logos\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/fhi.nl\/app\/uploads\/2024\/02\/Laboratorium-technologie.svg\" class=\"header__branch-logo\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/header>\n\n\t<div class=\"header__meta\">\n\t<div class=\"container\">\n\t\t<div class=\"header__meta__category\">\n\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Branch<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_branches_kennishub=laboratorium-technologie\" class=\"header__meta__detail--branch\">\n\t\t\t\t\t\t\t\tLaboratory Technology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/div>\n\n\n\n<div class=\"text bg--offwhite\">\n\t<div class=\"container\">\n\t\t\t\t\t\t<div class=\"text__content text__content--1-col\">\n\t\t\t<p><strong>Imagine: a test with the accuracy of PCR, but faster and simpler. Researchers from Eindhoven University of Technology have developed a new method for the detection of DNA\/RNA. PhD student Yosta de Stigter talks about the operation and application of this groundbreaking technology. <\/strong><\/p>\n<p>Polymerase Chain Reaction (PCR) is a reliable and widely used method for detecting bacteria and viruses. Through several cycles of heating and cooling, the DNA multiplies and tiny amounts of viral DNA are made detectable. PCR requires specialist equipment and must be carried out in the laboratory. In practice, this means that the patient has to wait one or more days for a test result. TU Eindhoven and Fontys University of Applied Sciences are working together on an alternative in which the GP makes the diagnosis himself and the patient receives the results within half an hour.<\/p>\n<p>Yosta is investigating the possibility of using this new method to test for STDs. \u201cThere is a taboo on the subject. Many people find it difficult to go to the GGD or the GP and ask for a test. It sometimes takes two days until you receive the test results, and during that time you cannot yet start a treatment plan. We hope to accelerate this process and lower the barrier to testing.\u201d<\/p>\n<p>But the researchers mainly see opportunities in countries where central laboratories are not always available. \u201cInfectious diseases, such as malaria, are still a major problem. Simple and cheap tests can really make a big difference.\u201d<\/p>\n<h4>LUNAS<\/h4>\n<p>Researchers from Eindhoven University of Technology have developed a Luminescent Nucleic Acid Sensor (LUNAS). LUNAS uses so-called <a href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acscentsci.2c01467\">luciferase enzymes<\/a>. These enzymes cause bioluminescence and thus emit light, such as in fireflies and glowworms. Yosta explains how it works: \u201cThe sensor detects the DNA of viruses or bacteria in a sample. To find the right DNA we use the D-Cas9 protein.\u201d These proteins are designed to detect and bind to a very specific piece of DNA, for example from a virus.<\/p>\n<p>\u201cThe luciferase enzymes help us detect the DNA,\u201d Yosta continues. \u201cWe split this into two pieces and attach it to two D-Cas9 proteins. The proteins look for the target DNA and bind to it. When this happens, the two pieces of luciferase enzyme come together and emit light. If the target DNA is not present, the D-Cas-9 proteins do not find each other and it remains dark.\u201d The scam is then made visible with a digital camera.<\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<td><em>The Cas9 protein is known from the CRISPR-Cas technique. This technique allows scientists to edit the DNA of organisms very precisely by cutting, replacing or adding pieces of genetic material. LUNAS uses the D-Cas9 protein. &#039;D&#039; stands for dead. The protein therefore no longer cuts, but only binds to the piece of DNA it is looking for<\/em><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4>Amplification<\/h4>\n<p>Finding a specific piece of DNA in a microliter sample is highly specialized work. \u201cYou can compare it to looking for one fish in Lake Victoria in Africa,\u201d says Yosta. \u201cPCR is so sensitive because it detects a tiny amount of DNA in a sample by amplifying it. The power of DNA is that we can amplify it, which is why DNA tests are the current standard in diagnostics.\u201d<\/p>\n<p>Yosta explains that the LUNAS method itself is not sensitive enough to detect clinically relevant samples, and is therefore combined with an amplification technique. The difference with PCR is that the researchers use an amplification technique that works at a single constant temperature. \u201cWe therefore only need two components, something that heats and a camera that can capture the light. This makes the test a lot easier and faster than a PCR test. The combination of speed and a simplified reading method means that in theory you can do this type of test at your GP.\u201d<\/p>\n<blockquote><p><em>\u201cThe combination of speed and a simplified reading method means that in theory you can do this type of test at your GP.\u201d<\/em><\/p><\/blockquote>\n<h4>From theory to practice<\/h4>\n<p>In theory, the LUNAS test can easily be performed at the GP, but to put this into practice, Yosta hopes to integrate the test into a microfluidics device. \u201cWe now work with a large box with a camera in it, but that is of course not a setup that the GP can work with. Moreover, it still requires a relatively large amount of laboratory work. We want to make the test more user-friendly.\u201d<\/p>\n<p>According to Yosta, it will certainly take a few more years before the first general practitioners can start using the LUNAS test. \u201cI don&#039;t know whether all general practitioners will be using exactly this test in ten years&#039; time, but I dare say that test results will become faster and diagnostics will change in the coming years.\u201d<\/p>\n\t\t<\/div>\n\t<\/div>\n<\/div>\n\n\r\n\t<div class=\"articles bg--dark-blue automatic\">\r\n\t\t<div class=\"container\">\r\n\t\t\t<div class=\"articles__header\">\r\n\t\t\t\t\t\t\t\t\t<div class='heading-wrapper'><svg width=\"13\" height=\"13\" viewbox=\"0 0 13 13\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><circle cx=\"6.394\" cy=\"6.5\" r=\"6.394\" fill=\"#000\"\/><\/svg><h2>Read more<\/h2><\/div>\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"\/en\/profiel\/fhi-federatie-van-technologie-branches\/\" class=\"button button--outline\">view profile<\/a>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t<div class=\"post-grid post-grid--no-padding\">\r\n\t\t\t\t\n<a class=\"single-item single-item__articles\" href=\"https:\/\/fhi.nl\/en\/news\/kort-economisch-coronanieuws-van-28-januari\/\" data-id=\"40016\">\n\t<div class=\"single-item__articles-icon\">\n\t\t<svg width=\"35\" height=\"35\" viewbox=\"0 0 35 35\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n<mask id=\"mask0_1182_5532\" style=\"mask-type:luminance\" maskunits=\"userSpaceOnUse\" x=\"0\" y=\"0\" width=\"35\" height=\"35\">\n<path d=\"M0 0H35V35H0V0Z\" fill=\"white\"\/>\n<\/mask>\n<g mask=\"url(#mask0_1182_5532)\">\n<path d=\"M5.12695 9.22852H1.02539V31.9238H10.4299C12.2868 31.9238 14.0678 32.6615 15.3809 33.9746H19.6191C20.9322 32.6615 22.7132 31.9238 24.5701 31.9238H33.9746V11.2793H29.873\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<path d=\"M17.5 9.22852H18.1426C20.0088 7.89544 22.2237 7.17773 24.5615 7.17773H29.873V27.8223H24.5615C22.2237 27.8223 20.0088 28.54 18.1426 29.873H16.8574C14.9912 28.54 12.7763 27.8223 10.4385 27.8223H5.12695V5.12695H9.22852\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<path d=\"M9.22852 1.02539V21.6699C13.759 21.6699 17.5 25.3425 17.5 29.873V9.22852C17.5 4.698 13.759 1.02539 9.22852 1.02539Z\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<\/g>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>Brief economic corona news from January 28<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Federated<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Economy<\/span>\n\t\t\t\t\t<\/div>\n\t<div class=\"single-item__articles-author-date-wrapper\">\n\t\t\t\t\t<div class=\"single-item__articles-author\">\n\t\t\t\tFHI, Federation of Technology Industries\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"single-item__articles-date\">\n\t\t\t\tJanuary 28, 2021\t\t\t<\/div>\n\t\t\t<\/div>\n<\/a>\n\n<a class=\"single-item single-item__articles\" href=\"https:\/\/fhi.nl\/en\/news\/extreem-dunne-batterijen\/\" data-id=\"43918\">\n\t<div class=\"single-item__articles-icon\">\n\t\t<svg width=\"35\" height=\"35\" viewbox=\"0 0 35 35\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n<mask id=\"mask0_1182_5532\" style=\"mask-type:luminance\" maskunits=\"userSpaceOnUse\" x=\"0\" y=\"0\" width=\"35\" height=\"35\">\n<path d=\"M0 0H35V35H0V0Z\" fill=\"white\"\/>\n<\/mask>\n<g mask=\"url(#mask0_1182_5532)\">\n<path d=\"M5.12695 9.22852H1.02539V31.9238H10.4299C12.2868 31.9238 14.0678 32.6615 15.3809 33.9746H19.6191C20.9322 32.6615 22.7132 31.9238 24.5701 31.9238H33.9746V11.2793H29.873\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<path d=\"M17.5 9.22852H18.1426C20.0088 7.89544 22.2237 7.17773 24.5615 7.17773H29.873V27.8223H24.5615C22.2237 27.8223 20.0088 28.54 18.1426 29.873H16.8574C14.9912 28.54 12.7763 27.8223 10.4385 27.8223H5.12695V5.12695H9.22852\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<path d=\"M9.22852 1.02539V21.6699C13.759 21.6699 17.5 25.3425 17.5 29.873V9.22852C17.5 4.698 13.759 1.02539 9.22852 1.02539Z\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<\/g>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>Extremely thin batteries<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Industrial Electronics<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline 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