{"id":35297,"date":"2022-07-26T10:45:00","date_gmt":"2022-07-26T08:45:00","guid":{"rendered":"https:\/\/fhi.nl\/nieuws\/s2-genomics-singulator-100\/"},"modified":"2024-07-10T13:24:00","modified_gmt":"2024-07-10T11:24:00","slug":"s2-genomics-singulator-100","status":"publish","type":"news","link":"https:\/\/fhi.nl\/en\/news\/s2-genomics-singulator-100\/","title":{"rendered":"S2 Genomics \u2013 Singulator 100"},"content":{"rendered":"
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\n\t\t\t\t\tS2 Genomics \u2013 Singulator 100\t\t\t\t<\/h1>\n\n\t\t\t\t
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Fast. High Yield. High Viability.<\/h2>\r\n\r\n\r\n\r\n\r\n

The Singulator\u2122 100 System<\/h2>\r\n

Solid Tissue Dissociation.
Automated.<\/p>\r\n

The bench-top Singulator System and its single-use cartridges enable reproducible, rapid and hands-off tissue dissociations into single-cell or nuclei suspensions.<\/p>\r\n

Researchers can now easily obtain suspensions of nuclei or high-viability cells for a wide range of single-cell analyses.<\/p>\r\n

\"Singulator<\/p>\r\n

\u00a0<\/p>\r\n

\u00a0<\/p>\r\n

\u00a0<\/p>\r\n

\u00a0<\/p>\r\n

\u00a0<\/p>\r\n

\u00a0<\/p>\r\n

Minimal Variability. Multiple Applications.<\/h2>\r\n

Ideal for genomics, cell biology and other 'omics applications, including scRNA-Seq, snRNA-Seq, ATAC-Seq, CITE-Seq, FACS, and immuno-oncology.
S2 Genomics provides a selection of pre-set protocols and pre-formulated reagents for cell isolations from an expanding set of mouse, rat, and human tissues, including tumors. See the wide range of tissues and organisms demonstrated on the Singulator 100 System for nuclei isolation at the end of this brochure.<\/p>\r\n

\"Applications\"<\/p>\r\n

\u00a0<\/p>\r\n

Process fresh, frozen and OCT tissues.<\/p>\r\n\r\n\r\n\r\n\r\n\r\n
\u00a0<\/td>\r\nTISSUE TYPE<\/strong><\/td>\r\nPROCESS TIME<\/strong><\/td>\r\nYIELD*<\/strong><\/td>\r\nVIABILITY*<\/strong><\/td>\r\n<\/tr>\r\n
Cells<\/td>\r\nFresh<\/td>\r\n20-60 minutes<\/td>\r\n10,000 to >500,000\/mg<\/td>\r\n70-95%<\/td>\r\n<\/tr>\r\n
Nuclei<\/td>\r\nFresh, Frozen, OCT<\/td>\r\n7-12 minutes<\/td>\r\n10,000 to >600,000\/mg<\/td>\r\nAFTER<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\n

*Varies depending on tissue types<\/p>\r\n

Nuclei In 7-12 Minutes<\/strong><\/h2>\r\n

Reproducibly generate high-quality suspensions of nuclei from a wide range of tissues.<\/p>\r\n

\"Singulator<\/p>\r\n

Merged DAPI-stained and bright-field images of small intestine, brain and heart tissue nuclei; DAPI stained liver nuclei. Courtesy of Dr. Minoda, Laboratory for Cellular Epigenomics, RIKEN Yokohama, Japan.<\/p>\r\n

High Yields From Small Samples<\/strong><\/h2>\r\n

\"Singulator<\/p>\r\n

Consistently High Yields of Nuclei<\/strong><\/h2>\r\n

\"Singulator<\/p>\r\n

See It In Action<\/strong><\/h2>\r\n

Watch this video to learn how to prepare single-nuclei suspension from mouse kidney tissue on the Singulator 100 System.<\/p>\r\n

<\/p>\r\n

Single Cells in 20-60 Minutes<\/strong><\/h2>\r\n

Reproducibly generate suspensions of single cells in high yield with high-viability from a wide range of tissues.<\/p>\r\n

\"Singulator<\/p>\r\n

Bright-field images of mouse kidney and spleen tissue cells.<\/em><\/p>\r\n

High Cell Yields<\/strong><\/h2>\r\n

\"Singulator<\/p>\r\n

High Cell Viabilities<\/strong><\/h2>\r\n

\"Singulator<\/p>\r\n

Intuitive Software. Customizable Protocols.<\/strong><\/h2>\r\n

Choose from a selection of pre-set protocols and pre-formulated reagents. Create your own protocols with customizable parameters, including mining, enzyme incubation time, temperature, mixing and mechanical disruption profile. Optionally, use your own reagents.<\/p>\r\n

\"Singulator<\/p>\r\n

1. Select from lists of Standard, Favorites, Recently Run protocols, or create your own protocol with customizable parameters.<\/p>\r\n

\"Singulator<\/p>\r\n

\"Singulator<\/p>\r\n

2. Enter optional user notes and press RUN.<\/p>\r\n

\"Singulator<\/p>\r\n

3. Step-by-step instructions and videos guide you through the system operation.<\/p>\r\n

\"Singulator<\/p>\r\n

4. The internal camera and progress bar allow you to monitor tissue dissociation in real time.<\/p>\r\n

\"Singulator<\/p>\r\n

Performance At A Glance<\/strong><\/h2>\r\n

The Singulator 100TM System comes with a unique Single-Shot MechanismTM for convenient cell-isolation reagent loading and a Reagent Chiller that stores reagents for up to 100 nuclei runs.<\/p>\r\n

\"Singulator<\/p>\r\n\r\n\t\t<\/div>\n\t<\/div>\n<\/div>\r\n\t

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Ideal for genomics, cell biology and other 'omics applications, including scRNA-Seq, snRNA-Seq, ATAC-Seq, CITE-Seq, FACS, and immuno-oncology.
\nS2 Genomics provides a selection of pre-set protocols and pre-formulated reagents for cell isolations from an expanding set of mouse, rat, and human tissues, including tumors. See the wide range of tissues and organisms demonstrated on the Singulator 100 System for nuclei isolation at the end of this brochure.<\/p>","protected":false},"featured_media":35298,"template":"","branches":[],"events":[8],"secretariat":[],"categories":[26,28,29,96,94,71,30,31,59,32,72,105,106,98],"themes_tax":[],"content_types":[514],"class_list":["post-35297","news","type-news","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"\nS2 Genomics Singulator 100<\/title>\n<meta name=\"description\" content=\"The bench-top Singulator System and its single-use cartridges enable reproducible, rapid and hands-off tissue dissociations into single-cell or nuclei suspensions.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/fhi.nl\/en\/nieuws\/s2-genomics-singulator-100\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" 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