{"id":35557,"date":"2022-06-29T11:11:00","date_gmt":"2022-06-29T09:11:00","guid":{"rendered":"https:\/\/fhi.nl\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/"},"modified":"2024-07-10T13:25:27","modified_gmt":"2024-07-10T11:25:27","slug":"bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor","status":"publish","type":"news","link":"https:\/\/fhi.nl\/en\/news\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/","title":{"rendered":"Bio Rad. Troubleshooting Western Blots with the Western Blot Doctor\u2122"},"content":{"rendered":"<header id=\"header\" class=\"header header--low header--branch\">\n\n\t\n\t\t\t<div class=\"header__background header__background--graphic\"><\/div>\n\t\n\t<div class=\"container\">\n\t\t<div class=\"header__content\">\n\t\t\t<div class=\"header__first\">\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<h1 class=\"header__title\" >\n\t\t\t\t\tBio Rad. Troubleshooting Western Blots with the Western Blot Doctor\u2122\t\t\t\t<\/h1>\n\n\t\t\t\t<div class=\"header__dots-line\">\n\t\t\t\t\t<svg width=\"431\" height=\"9\" viewbox=\"0 0 431 9\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M430.799 4.192a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.135 1.135 0 1 1-2.27 0 1.135 1.135 0 0 1 2.27 0Zm-27.27 0a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.39 1.39 0 1 1-2.78 0 1.39 1.39 0 0 1 2.78 0Zm-27.78 0a1.645 1.645 0 1 1-3.29 0 1.645 1.645 0 0 1 3.29 0Zm-28.29 0a1.9 1.9 0 1 1-3.799 0 1.9 1.9 0 0 1 3.799 0Zm-28.799 0a2.154 2.154 0 1 1-4.308 0 2.154 2.154 0 0 1 4.308 0Zm-29.308 0a2.41 2.41 0 1 1-4.819 0 2.41 2.41 0 0 1 4.819 0Zm-29.819 0a2.663 2.663 0 1 1-5.326.001 2.663 2.663 0 0 1 5.326-.001Zm-30.327 0a2.919 2.919 0 1 1-5.837 0 2.919 2.919 0 0 1 5.837 0Zm-30.837 0a3.173 3.173 0 1 1-6.345.001 3.173 3.173 0 0 1 6.345 0Zm-31.346 0a3.428 3.428 0 1 1-6.856 0 3.428 3.428 0 0 1 6.856 0Zm-31.856 0a3.683 3.683 0 1 1-7.365 0 3.683 3.683 0 0 1 7.365 0Zm-32.365 0a3.937 3.937 0 1 1-7.875 0 3.937 3.937 0 0 1 7.875 0Zm-32.874 0a4.192 4.192 0 1 1-8.384 0 4.192 4.192 0 0 1 8.384 0Z\" fill=\"#FFF960\"\/><\/svg>\t\t\t\t<\/div>\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"header__second\">\n\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"header__branch-logos\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/fhi.nl\/app\/uploads\/2024\/02\/Laboratorium-technologie.svg\" class=\"header__branch-logo\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/header>\n\n\t<div class=\"header__meta header__meta--footer\">\n\t<div class=\"container\">\n\t\t<div class=\"header__meta__category\">\n\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Branch<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_branches_kennishub=laboratorium-technologie\" class=\"header__meta__detail--branch\">\n\t\t\t\t\t\t\t\tLaboratory Technology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Subject<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=algemene-laboratorium-benodigdheden\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tGeneral laboratory supplies\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=analytische-instrumenten\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tAnalytical Instruments\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=consumables\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tConsumables\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=elektroforese\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tElectrophoresis\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=image-analysers\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tImage analyzers\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=laboratorium-technologie\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tLaboratory Technology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=life-science-biotechnologie\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tLife Science\/Biotechnology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=moleculaire-biologie\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tMolecular biology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/div>\n\n<div class=\"text bg--white\">\n\t<div class=\"container\">\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"text__content text__content--1-col\">\n\t\t\t<p><strong>The Western Blot Doctor is a self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems. Comprehensive solutions and suggestions are provided to help solve your particular western blotting challenges.with the Western Blot Doctor\u2122\u00a0<\/strong><\/p>\r\n<p><strong><img decoding=\"async\" src=\"https:\/\/fhi.nl\/app\/uploads\/filemanager\/1875\/1656494137_wb doctor.jpg\" alt=\"\" width=\"310\" height=\"206\" \/><\/strong><\/p>\r\n<p>\u00a0<\/p>\r\n<p>Tips, Techniques, and Technologies from the Western Blotting Experts at Bio\u2011Rad Laboratories: \u00a0<a href=\"https:\/\/info.bio-rad.com\/ww-Western-Blotting-Guide-lp1.html\">Get Your Free Guide<\/a><\/p>\r\n<p>The Western Blot Doctor is a self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems. Comprehensive solutions and suggestions are provided to help solve your particular western blotting challenges.<\/p>\r\n<p>To start troubleshooting your western blotting problems, choose the type of problem you are experiencing.<\/p>\r\n<p><strong>I am having trouble with:<\/strong><\/p>\r\n<p><strong><a href=\"https:\/\/www.bio-rad.com\/evportal\/destination\/solutions?catID=MIW4Q8C4S\">Protein Band Appearance <\/a><\/strong><\/p>\r\n<ul>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-appearance-problems#inconsistentcontrol\">Inconsistent control protein levels across samples<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-appearance-problems#ghostprotein\">Ghost protein bands<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-appearance-problems#swirls\">Swirls or missing bands; bands appear diffuse on blot<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-appearance-problems#blurrybands\">Blurry bands<\/a><\/li>\r\n<\/ul>\r\n<ul>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-appearance-problems#smiling\">Bands are curved (smiling) not straight<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-appearance-problems#broadband\">Broad or misshapen bands<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-appearance-problems#whitebands\">White (negative) bands on film using ECL method<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-appearance-problems#nobands\">No bands<\/a><\/li>\r\n<\/ul>\r\n<p><strong><a href=\"https:\/\/www.bio-rad.com\/evportal\/destination\/solutions?catID=MIW4S08UU\">Signal Strength<\/a><\/strong><\/p>\r\n<ul>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-signal-strength-problems#uneven\">Uneven signal across blot<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-signal-strength-problems#targetsignal\">Saturated band signal<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-signal-strength-problems#fainthighmwbands\">Faint high-MW bands<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-signal-strength-problems#weaknosignal\">Faint bands, weak or no signal<\/a><\/li>\r\n<\/ul>\r\n<p><strong><a href=\"https:\/\/www.bio-rad.com\/evportal\/destination\/solutions?catID=MIW4MCKG4\">Protein Band Size and Pattern<\/a><\/strong><\/p>\r\n<ul>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-size-pattern-problems#lowermw\">Band(s) at lower MW than expected<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-size-pattern-problems#slightlyhighermw\">Band(s) at slightly higher MW than expected, and may be blurry<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-size-pattern-problems#significantlyhighermw\">Band(s) at significantly higher MW than expected<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-protein-band-size-pattern-problems#uneventransfer\">Multiple bands at various MWs<\/a><\/li>\r\n<\/ul>\r\n<p><strong><a href=\"https:\/\/www.bio-rad.com\/evportal\/destination\/solutions?catID=MIW4O8ESH\">Blot Background <\/a><\/strong><\/p>\r\n<ul>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-blot-background-problems#whitespots\">White spots or regions on membrane<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-blot-background-problems#highbackground\">High background on blot<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-blot-background-problems#blotchybackground\">Blotchy or patchy background<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-blot-background-problems#speckledbackground\">Uneven spots on blot or speckled background<\/a><\/li>\r\n<li><a href=\"https:\/\/www.bio-rad.com\/en-us\/applications-technologies\/western-blot-doctor-blot-background-problems#tankblotters\">Shadow of tank blotter gel cassette on blot<\/a><\/li>\r\n<\/ul>\r\n<p>For more information visit us at booth 7C074<\/p>\t\t<\/div>\n\t<\/div>\n<\/div>\r\n\t<div class=\"articles bg--offwhite automatic\">\r\n\t\t<div class=\"container\">\r\n\t\t\t<div class=\"articles__header\">\r\n\t\t\t\t\t\t\t\t\t<div class='heading-wrapper'><svg width=\"13\" height=\"13\" viewbox=\"0 0 13 13\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><circle cx=\"6.394\" cy=\"6.5\" r=\"6.394\" fill=\"#000\"\/><\/svg><h2>Related articles<\/h2><\/div>\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"\/en\/profiel\/bio-rad-laboratories\/\" class=\"button button--outline\">view profile<\/a>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t<div class=\"post-grid post-grid--no-padding\">\r\n\t\t\t\t\n<a class=\"single-item single-item__articles\" href=\"https:\/\/fhi.nl\/en\/news\/themadag-werkruimtes-en-autoclaven\/\" data-id=\"50511\">\n\t<div class=\"single-item__articles-icon\">\n\t\t<svg width=\"82\" height=\"98\" viewbox=\"0 0 82 98\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n<path fill-rule=\"evenodd\" clip-rule=\"evenodd\" d=\"M26.5586 7.96501C26.5586 6.61879 26.8909 5.49682 27.3233 4.77796C27.7792 4.02009 28.1318 4 28.1466 4H55.0564C55.0712 4 55.4238 4.02009 55.8796 4.77796C56.312 5.49682 56.6444 6.61879 56.6444 7.96501C56.6444 9.31122 56.312 10.4332 55.8796 11.1521C55.4238 11.9099 55.0712 11.93 55.0564 11.93H54.2487C53.8841 11.5449 53.3681 11.3047 52.796 11.3047H28.981C28.5233 11.3047 28.1015 11.4584 27.7644 11.7171C27.6381 11.602 27.4874 11.4248 27.3233 11.1521C26.8909 10.4332 26.5586 9.31122 26.5586 7.96501ZM26.981 15.7683C25.6024 15.3755 24.5619 14.3215 23.8956 13.2138C23.0294 11.7738 22.5586 9.91326 22.5586 7.96501C22.5586 6.01675 23.0294 4.15622 23.8956 2.71619C24.7383 1.31517 26.1797 0 28.1466 0H55.0564C57.0232 0 58.4646 1.31517 59.3073 2.71619C60.1735 4.15622 60.6444 6.01675 60.6444 7.96501C60.6444 9.91326 60.1735 11.7738 59.3073 13.2138C58.4646 14.6148 57.0232 15.93 55.0564 15.93H54.796V40.3243L80.5327 84.0062L80.5391 84.0172L80.5503 84.0368C84.0711 90.2609 79.5954 97.9997 72.4362 97.9997H9.34052C2.18152 97.9997 -2.29415 90.2608 1.22665 84.0368C1.23243 84.0265 1.23831 84.0163 1.24428 84.0062L26.981 40.3243V15.7683ZM30.981 15.93V35.792H39.161C40.2655 35.792 41.161 36.6874 41.161 37.792C41.161 38.8966 40.2655 39.792 39.161 39.792H30.981V40.8697C30.981 41.2268 30.8854 41.5773 30.7041 41.8849L21.3184 57.8149H60.4585L51.0728 41.8849C50.8916 41.5773 50.796 41.2268 50.796 40.8697V15.93H30.981ZM62.8152 61.8148C62.8065 61.8149 62.7978 61.8149 62.7891 61.8149H19.4727C19.3082 61.8149 19.1484 61.7951 18.9954 61.7577L4.70016 86.0205C2.69212 89.5942 5.27194 93.9997 9.34052 93.9997H72.4362C76.5051 93.9997 79.0848 89.5941 77.0768 86.0205L62.8152 61.8148Z\" fill=\"#FFDA56\"\/>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>Theme day Workspaces and Autoclaves<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Laboratory Technology<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">World of Industry, Technology &amp; Science<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">General laboratory equipment<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Life Science\/Biotechnology<\/span>\n\t\t\t\t\t<\/div>\n\t<div class=\"single-item__articles-author-date-wrapper\">\n\t\t\t\t\t<div class=\"single-item__articles-author\">\n\t\t\t\tSALM AND KIPP\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"single-item__articles-date\">\n\t\t\t\tSeptember 17, 2024\t\t\t<\/div>\n\t\t\t<\/div>\n<\/a>\n\n<a class=\"single-item single-item__articles\" href=\"https:\/\/fhi.nl\/en\/news\/bewustzijn-cyber-security-neemt-toe-maar-veel-basisstappen-zijn-nog-nodig-in-de-sector\/\" data-id=\"36169\">\n\t<div class=\"single-item__articles-icon\">\n\t\t<svg width=\"82\" height=\"98\" viewbox=\"0 0 82 98\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n<path fill-rule=\"evenodd\" clip-rule=\"evenodd\" d=\"M26.5586 7.96501C26.5586 6.61879 26.8909 5.49682 27.3233 4.77796C27.7792 4.02009 28.1318 4 28.1466 4H55.0564C55.0712 4 55.4238 4.02009 55.8796 4.77796C56.312 5.49682 56.6444 6.61879 56.6444 7.96501C56.6444 9.31122 56.312 10.4332 55.8796 11.1521C55.4238 11.9099 55.0712 11.93 55.0564 11.93H54.2487C53.8841 11.5449 53.3681 11.3047 52.796 11.3047H28.981C28.5233 11.3047 28.1015 11.4584 27.7644 11.7171C27.6381 11.602 27.4874 11.4248 27.3233 11.1521C26.8909 10.4332 26.5586 9.31122 26.5586 7.96501ZM26.981 15.7683C25.6024 15.3755 24.5619 14.3215 23.8956 13.2138C23.0294 11.7738 22.5586 9.91326 22.5586 7.96501C22.5586 6.01675 23.0294 4.15622 23.8956 2.71619C24.7383 1.31517 26.1797 0 28.1466 0H55.0564C57.0232 0 58.4646 1.31517 59.3073 2.71619C60.1735 4.15622 60.6444 6.01675 60.6444 7.96501C60.6444 9.91326 60.1735 11.7738 59.3073 13.2138C58.4646 14.6148 57.0232 15.93 55.0564 15.93H54.796V40.3243L80.5327 84.0062L80.5391 84.0172L80.5503 84.0368C84.0711 90.2609 79.5954 97.9997 72.4362 97.9997H9.34052C2.18152 97.9997 -2.29415 90.2608 1.22665 84.0368C1.23243 84.0265 1.23831 84.0163 1.24428 84.0062L26.981 40.3243V15.7683ZM30.981 15.93V35.792H39.161C40.2655 35.792 41.161 36.6874 41.161 37.792C41.161 38.8966 40.2655 39.792 39.161 39.792H30.981V40.8697C30.981 41.2268 30.8854 41.5773 30.7041 41.8849L21.3184 57.8149H60.4585L51.0728 41.8849C50.8916 41.5773 50.796 41.2268 50.796 40.8697V15.93H30.981ZM62.8152 61.8148C62.8065 61.8149 62.7978 61.8149 62.7891 61.8149H19.4727C19.3082 61.8149 19.1484 61.7951 18.9954 61.7577L4.70016 86.0205C2.69212 89.5942 5.27194 93.9997 9.34052 93.9997H72.4362C76.5051 93.9997 79.0848 89.5941 77.0768 86.0205L62.8152 61.8148Z\" fill=\"#FFDA56\"\/>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>\u201cCyber security awareness is increasing, but many basic steps are still needed in the sector\u201d<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Laboratory Technology<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">LabSafety<\/span>\n\t\t\t\t\t<\/div>\n\t<div class=\"single-item__articles-author-date-wrapper\">\n\t\t\t\t\t<div class=\"single-item__articles-author\">\n\t\t\t\tFHI, Federation of Technology Industries\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"single-item__articles-date\">\n\t\t\t\tApril 21, 2022\t\t\t<\/div>\n\t\t\t<\/div>\n<\/a>\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/div>","protected":false},"excerpt":{"rendered":"<p>The Western Blot Doctor is a self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems. Comprehensive solutions and suggestions are provided to help solve your particular western blotting challenges.<\/p>","protected":false},"featured_media":35558,"template":"","branches":[14],"events":[8],"secretariat":[],"categories":[27,28,81,87,103,59,32,105],"themes_tax":[],"content_types":[514],"class_list":["post-35557","news","type-news","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems<\/title>\n<meta name=\"description\" content=\"Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/fhi.nl\/en\/wots\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems\" \/>\n<meta property=\"og:description\" content=\"Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems\" \/>\n<meta property=\"og:url\" content=\"https:\/\/fhi.nl\/en\/wots\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/\" \/>\n<meta property=\"og:site_name\" content=\"FHI, federatie van technologiebranches\" \/>\n<meta property=\"article:modified_time\" content=\"2024-07-10T11:25:27+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/fhi.nl\/app\/uploads\/news\/69869c934b8d9053271e5bc202e149b9-wb-doctor.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"247\" \/>\n\t<meta property=\"og:image:height\" content=\"164\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/fhi.nl\\\/nieuws\\\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\\\/\",\"url\":\"https:\\\/\\\/fhi.nl\\\/wots\\\/nieuws\\\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\\\/\",\"name\":\"Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/fhi.nl\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/fhi.nl\\\/wots\\\/nieuws\\\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/fhi.nl\\\/wots\\\/nieuws\\\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/fhi.nl\\\/app\\\/uploads\\\/news\\\/69869c934b8d9053271e5bc202e149b9-wb-doctor.jpg\",\"datePublished\":\"2022-06-29T09:11:00+00:00\",\"dateModified\":\"2024-07-10T11:25:27+00:00\",\"description\":\"Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/fhi.nl\\\/wots\\\/nieuws\\\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\\\/#breadcrumb\"},\"inLanguage\":\"en-GB\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/fhi.nl\\\/wots\\\/nieuws\\\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\\\/\\\/fhi.nl\\\/wots\\\/nieuws\\\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\\\/#primaryimage\",\"url\":\"https:\\\/\\\/fhi.nl\\\/app\\\/uploads\\\/news\\\/69869c934b8d9053271e5bc202e149b9-wb-doctor.jpg\",\"contentUrl\":\"https:\\\/\\\/fhi.nl\\\/app\\\/uploads\\\/news\\\/69869c934b8d9053271e5bc202e149b9-wb-doctor.jpg\",\"width\":247,\"height\":164},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/fhi.nl\\\/wots\\\/nieuws\\\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/fhi.nl\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Nieuws\",\"item\":\"https:\\\/\\\/fhi.nl\\\/nieuws\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Bio-Rad. Troubleshooting Western Blots with the Western Blot Doctor\u2122\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/fhi.nl\\\/#website\",\"url\":\"https:\\\/\\\/fhi.nl\\\/\",\"name\":\"FHI, federatie van technologiebranches\",\"description\":\"Nederlandse branchevereniging voor technologiebranches\",\"publisher\":{\"@id\":\"https:\\\/\\\/fhi.nl\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/fhi.nl\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-GB\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/fhi.nl\\\/#organization\",\"name\":\"FHI, federatie van technologiebranches\",\"url\":\"https:\\\/\\\/fhi.nl\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-GB\",\"@id\":\"https:\\\/\\\/fhi.nl\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/fhi.nl\\\/app\\\/uploads\\\/2024\\\/06\\\/3-e1722349014385.png\",\"contentUrl\":\"https:\\\/\\\/fhi.nl\\\/app\\\/uploads\\\/2024\\\/06\\\/3-e1722349014385.png\",\"width\":732,\"height\":136,\"caption\":\"FHI, federatie van technologiebranches\"},\"image\":{\"@id\":\"https:\\\/\\\/fhi.nl\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/www.linkedin.com\\\/company\\\/fhi-federation-of-technology-branches\",\"https:\\\/\\\/www.instagram.com\\\/fhi_nl\\\/\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems","description":"Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/fhi.nl\/en\/wots\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/","og_locale":"en_GB","og_type":"article","og_title":"Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems","og_description":"Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems","og_url":"https:\/\/fhi.nl\/en\/wots\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/","og_site_name":"FHI, federatie van technologiebranches","article_modified_time":"2024-07-10T11:25:27+00:00","og_image":[{"width":247,"height":164,"url":"https:\/\/fhi.nl\/app\/uploads\/news\/69869c934b8d9053271e5bc202e149b9-wb-doctor.jpg","type":"image\/jpeg"}],"twitter_card":"summary_large_image","schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/fhi.nl\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/","url":"https:\/\/fhi.nl\/wots\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/","name":"Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems","isPartOf":{"@id":"https:\/\/fhi.nl\/#website"},"primaryImageOfPage":{"@id":"https:\/\/fhi.nl\/wots\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/#primaryimage"},"image":{"@id":"https:\/\/fhi.nl\/wots\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/#primaryimage"},"thumbnailUrl":"https:\/\/fhi.nl\/app\/uploads\/news\/69869c934b8d9053271e5bc202e149b9-wb-doctor.jpg","datePublished":"2022-06-29T09:11:00+00:00","dateModified":"2024-07-10T11:25:27+00:00","description":"Self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems","breadcrumb":{"@id":"https:\/\/fhi.nl\/wots\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https:\/\/fhi.nl\/wots\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/"]}]},{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/fhi.nl\/wots\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/#primaryimage","url":"https:\/\/fhi.nl\/app\/uploads\/news\/69869c934b8d9053271e5bc202e149b9-wb-doctor.jpg","contentUrl":"https:\/\/fhi.nl\/app\/uploads\/news\/69869c934b8d9053271e5bc202e149b9-wb-doctor.jpg","width":247,"height":164},{"@type":"BreadcrumbList","@id":"https:\/\/fhi.nl\/wots\/nieuws\/bio-rad-troubleshooting-western-blots-with-the-western-blot-doctor\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/fhi.nl\/"},{"@type":"ListItem","position":2,"name":"Nieuws","item":"https:\/\/fhi.nl\/nieuws\/"},{"@type":"ListItem","position":3,"name":"Bio-Rad. Troubleshooting Western Blots with the Western Blot Doctor\u2122"}]},{"@type":"WebSite","@id":"https:\/\/fhi.nl\/#website","url":"https:\/\/fhi.nl\/","name":"FHI, federation of technology industries","description":"Dutch trade association for technology industries","publisher":{"@id":"https:\/\/fhi.nl\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/fhi.nl\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-GB"},{"@type":"Organization","@id":"https:\/\/fhi.nl\/#organization","name":"FHI, federation of technology industries","url":"https:\/\/fhi.nl\/","logo":{"@type":"ImageObject","inLanguage":"en-GB","@id":"https:\/\/fhi.nl\/#\/schema\/logo\/image\/","url":"https:\/\/fhi.nl\/app\/uploads\/2024\/06\/3-e1722349014385.png","contentUrl":"https:\/\/fhi.nl\/app\/uploads\/2024\/06\/3-e1722349014385.png","width":732,"height":136,"caption":"FHI, federatie van technologiebranches"},"image":{"@id":"https:\/\/fhi.nl\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.linkedin.com\/company\/fhi-federation-of-technology-branches","https:\/\/www.instagram.com\/fhi_nl\/"]}]}},"_links":{"self":[{"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/news\/35557","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/news"}],"about":[{"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/types\/news"}],"version-history":[{"count":0,"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/news\/35557\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/media\/35558"}],"wp:attachment":[{"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/media?parent=35557"}],"wp:term":[{"taxonomy":"branches","embeddable":true,"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/branches?post=35557"},{"taxonomy":"events","embeddable":true,"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/events?post=35557"},{"taxonomy":"secretariat","embeddable":true,"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/secretariat?post=35557"},{"taxonomy":"categories","embeddable":true,"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/categories?post=35557"},{"taxonomy":"themes","embeddable":true,"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/themes_tax?post=35557"},{"taxonomy":"content_types","embeddable":true,"href":"https:\/\/fhi.nl\/en\/wp-json\/wp\/v2\/content_types?post=35557"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}