{"id":76025,"date":"2025-11-24T14:11:00","date_gmt":"2025-11-24T13:11:00","guid":{"rendered":"https:\/\/fhi.nl\/nieuws\/sneller-van-idee-naar-eiwit-zo-haal-je-het-maximale-uit-je-workflow\/"},"modified":"2025-11-24T14:29:34","modified_gmt":"2025-11-24T13:29:34","slug":"sneller-van-idee-naar-eiwit-zo-haal-je-het-maximale-uit-je-workflow","status":"publish","type":"news","link":"https:\/\/fhi.nl\/en\/news\/sneller-van-idee-naar-eiwit-zo-haal-je-het-maximale-uit-je-workflow\/","title":{"rendered":"From idea to protein faster. How to get the most out of your workflow."},"content":{"rendered":"<header id=\"header\" class=\"header header--high header--branch\">\n\n\t\t\t\t\t\t\t\t\t\t<div class=\"header__background header__background--high\">\n\t\t\t\t\t<img decoding=\"async\" class=\"header__background-image\" src=\"https:\/\/fhi.nl\/app\/uploads\/2025\/11\/insp.png\" alt=\"\">\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\n\t\n\t<div class=\"container\">\n\t\t<div class=\"header__content\">\n\t\t\t<div class=\"header__first\">\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<h1 class=\"header__title\" >\n\t\t\t\t\tFrom idea to protein faster. How to get the most out of your workflow.\t\t\t\t<\/h1>\n\n\t\t\t\t<div class=\"header__dots-line\">\n\t\t\t\t\t<svg width=\"431\" height=\"9\" viewbox=\"0 0 431 9\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M430.799 4.192a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.135 1.135 0 1 1-2.27 0 1.135 1.135 0 0 1 2.27 0Zm-27.27 0a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.39 1.39 0 1 1-2.78 0 1.39 1.39 0 0 1 2.78 0Zm-27.78 0a1.645 1.645 0 1 1-3.29 0 1.645 1.645 0 0 1 3.29 0Zm-28.29 0a1.9 1.9 0 1 1-3.799 0 1.9 1.9 0 0 1 3.799 0Zm-28.799 0a2.154 2.154 0 1 1-4.308 0 2.154 2.154 0 0 1 4.308 0Zm-29.308 0a2.41 2.41 0 1 1-4.819 0 2.41 2.41 0 0 1 4.819 0Zm-29.819 0a2.663 2.663 0 1 1-5.326.001 2.663 2.663 0 0 1 5.326-.001Zm-30.327 0a2.919 2.919 0 1 1-5.837 0 2.919 2.919 0 0 1 5.837 0Zm-30.837 0a3.173 3.173 0 1 1-6.345.001 3.173 3.173 0 0 1 6.345 0Zm-31.346 0a3.428 3.428 0 1 1-6.856 0 3.428 3.428 0 0 1 6.856 0Zm-31.856 0a3.683 3.683 0 1 1-7.365 0 3.683 3.683 0 0 1 7.365 0Zm-32.365 0a3.937 3.937 0 1 1-7.875 0 3.937 3.937 0 0 1 7.875 0Zm-32.874 0a4.192 4.192 0 1 1-8.384 0 4.192 4.192 0 0 1 8.384 0Z\" fill=\"#FFF960\"\/><\/svg>\t\t\t\t<\/div>\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"header__second\">\n\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"header__branch-logos\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/fhi.nl\/app\/uploads\/2024\/02\/Laboratorium-technologie.svg\" class=\"header__branch-logo\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/header>\n\n\t<div class=\"header__meta\">\n\t<div class=\"container\">\n\t\t<div class=\"header__meta__category\">\n\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Branch<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_branches_kennishub=laboratorium-technologie\" class=\"header__meta__detail--branch\">\n\t\t\t\t\t\t\t\tLaboratory Technology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Subject<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=laboratorium-technologie\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tLaboratory Technology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=life-science-biotechnologie\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tLife Science\/Biotechnology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/div>\n\n<div class=\"text bg--white\">\n\t<div class=\"container\">\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"text__content text__content--1-col\">\n\t\t\t<p>Classical cell-based protein expression is time-consuming. Cloning, transformation, expression, and purification often take several days. Furthermore, some constructs can be toxic in cells, hindering your workflow.<\/p>\r\n<p>Cell-free protein synthesis (CFPS) makes this much faster. By first assembling DNA and then using it directly in a cell-free system, you can produce proteins the same day. This enables rapid variant screening and is ideal for synthetic biology and protein engineering.<\/p>\r\n<p>In this blog post, we demonstrate how to streamline the cell-free workflow from design to purified protein. This approach shortens the traditional cell-based workflow from seven days to one and is ideally suited for automation, increased throughput, and increased productivity.<\/p>\r\n<h3 data-start=\"725\" data-end=\"760\">1. Design your constructs smartly<\/h3>\r\n<p data-start=\"761\" data-end=\"832\">New England Biolabs (NEB) offers free tools that simplify the design process. These include:<\/p>\r\n<ul data-start=\"834\" data-end=\"1024\">\r\n<li data-start=\"834\" data-end=\"882\">\r\n<p data-start=\"836\" data-end=\"882\">NEBuilder Assembly Tool for HiFi assembly<\/p>\r\n<\/li>\r\n<li data-start=\"883\" data-end=\"956\">\r\n<p data-start=\"885\" data-end=\"956\">NEBridge Golden Gate Assembly Tool for Type IIS-based designs<\/p>\r\n<\/li>\r\n<li data-start=\"957\" data-end=\"1024\">\r\n<p data-start=\"959\" data-end=\"1024\">NEBridge Ligase Fidelity Tools for complex, modular projects<\/p>\r\n<\/li>\r\n<\/ul>\r\n<p data-start=\"1026\" data-end=\"1108\">These tools aid in primer design and increase your chances of successful assembly.<\/p>\r\n<h3 data-start=\"1110\" data-end=\"1139\">2. Create and purify your DNA<\/h3>\r\n<p data-start=\"1140\" data-end=\"1339\">For best results, use PCR amplicons or sheared DNA fragments and purify your DNA, for example, with Monarch PCR &amp; DNA Cleanup kits from NEB. High-quality input is essential for stable assembly and expression.<\/p>\r\n<h3 data-start=\"1341\" data-end=\"1372\">3. Assemble your DNA<\/h3>\r\n<p>Depending on your project, you choose:<\/p>\r\n<ul>\r\n<li>NEBuilder HiFi DNA Assembly Master Mix for seamless assembly and parallel variations<\/li>\r\n<li>NEBridge Golden Gate Assembly Kit for fast, modular assembly of 2 to more than 50 fragments<\/li>\r\n<\/ul>\r\n<p>These high-fidelity and high-efficiency assembly methods yield products that can be used directly in Rolling Circle Amplification (RCA), after which they serve as a template for Cell-free Protein Synthesis (CFPS).<\/p>\r\n<p>NEBuilder HiFi DNA Assembly is suitable for seamless DNA assembly regardless of fragment length or end compatibility. The method is highly accurate, allowing parallel assemblies to be easily scaled up. Ideal for prototyping protein variants for engineering and screening.<\/p>\r\n<h3 data-start=\"1669\" data-end=\"1702\">4. Multiply your DNA with RCA<\/h3>\r\n<p data-start=\"1703\" data-end=\"1898\">CFPS often requires more DNA. Rolling Circle Amplification allows you to generate large quantities of circular DNA within 2 hours. Only successfully ligated constructs are amplified.<\/p>\r\n<h3 data-start=\"1900\" data-end=\"1934\">5. Cell-free Protein Synthesis (CFPS)<\/h3>\r\n<p data-start=\"1935\" data-end=\"2147\">The NEBExpress Cell-free E. coli Protein Synthesis System allows you to synthesize proteins, including difficult or toxic proteins, in 2 to 4 hours. The system is scalable and suitable for automation.<\/p>\r\n<h3 data-start=\"2149\" data-end=\"2172\">6. Purify your protein<\/h3>\r\n<p data-start=\"2173\" data-end=\"2357\">Use magnetic beads from NEBExpress Ni NTA, SNAP Capture, or amylose beads to rapidly isolate His-tag, SNAP-tag, or MBP fusions. This works for both low-volume and high-throughput applications.<\/p>\r\n<h3 data-start=\"2359\" data-end=\"2390\">Why this workflow works<\/h3>\r\n<ul data-start=\"2391\" data-end=\"2558\">\r\n<li data-start=\"2391\" data-end=\"2414\">\r\n<p data-start=\"2393\" data-end=\"2414\">from days to hours<\/p>\r\n<\/li>\r\n<li data-start=\"2415\" data-end=\"2448\">\r\n<p data-start=\"2417\" data-end=\"2448\">ideal for variant screening<\/p>\r\n<\/li>\r\n<li data-start=\"2449\" data-end=\"2482\">\r\n<p data-start=\"2451\" data-end=\"2482\">compatible with automation<\/p>\r\n<\/li>\r\n<li data-start=\"2483\" data-end=\"2521\">\r\n<p data-start=\"2485\" data-end=\"2521\">cost reduction in miniaturization<\/p>\r\n<\/li>\r\n<li data-start=\"2522\" data-end=\"2558\">\r\n<p data-start=\"2524\" data-end=\"2558\">also works for challenging proteins<\/p>\r\n<\/li>\r\n<\/ul>\r\n<p data-start=\"2560\" data-end=\"2629\" data-is-last-node=\"\" data-is-only-node=\"\"><a href=\"https:\/\/www.neb.com\/en\/nebinspired-blog\/design-assemble-and-express-with-confidence\">For the full blog in English, please visit the NEB website.<\/a><\/p>\t\t<\/div>\n\t<\/div>\n<\/div>\r\n\t<div class=\"articles bg--offwhite automatic\">\r\n\t\t<div class=\"container\">\r\n\t\t\t<div class=\"articles__header\">\r\n\t\t\t\t\t\t\t\t\t<div class='heading-wrapper'><svg width=\"13\" height=\"13\" viewbox=\"0 0 13 13\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><circle cx=\"6.394\" cy=\"6.5\" r=\"6.394\" fill=\"#000\"\/><\/svg><h2>Related articles<\/h2><\/div>\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"\/en\/profiel\/cell-signaling-technology\/\" class=\"button button--outline\">view profile<\/a>\r\n\t\t\t\t\t\t\t<\/div>\r\n\t\t\t\t\t\t<div class=\"post-grid post-grid--no-padding\">\r\n\t\t\t\t\n<a class=\"single-item single-item__articles\" href=\"https:\/\/fhi.nl\/en\/news\/exposant-uitgelicht-distrelec\/\" data-id=\"40468\">\n\t<div class=\"single-item__articles-icon\">\n\t\t<svg width=\"35\" height=\"35\" viewbox=\"0 0 35 35\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n<mask id=\"mask0_1182_5532\" style=\"mask-type:luminance\" maskunits=\"userSpaceOnUse\" x=\"0\" y=\"0\" width=\"35\" height=\"35\">\n<path d=\"M0 0H35V35H0V0Z\" fill=\"white\"\/>\n<\/mask>\n<g mask=\"url(#mask0_1182_5532)\">\n<path d=\"M5.12695 9.22852H1.02539V31.9238H10.4299C12.2868 31.9238 14.0678 32.6615 15.3809 33.9746H19.6191C20.9322 32.6615 22.7132 31.9238 24.5701 31.9238H33.9746V11.2793H29.873\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<path d=\"M17.5 9.22852H18.1426C20.0088 7.89544 22.2237 7.17773 24.5615 7.17773H29.873V27.8223H24.5615C22.2237 27.8223 20.0088 28.54 18.1426 29.873H16.8574C14.9912 28.54 12.7763 27.8223 10.4385 27.8223H5.12695V5.12695H9.22852\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<path d=\"M9.22852 1.02539V21.6699C13.759 21.6699 17.5 25.3425 17.5 29.873V9.22852C17.5 4.698 13.759 1.02539 9.22852 1.02539Z\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<\/g>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>Featured exhibitor: Distrelec<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Industrial Electronics<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Medical Technology<\/span>\n\t\t\t\t\t<\/div>\n\t<div class=\"single-item__articles-author-date-wrapper\">\n\t\t\t\t\t<div class=\"single-item__articles-author\">\n\t\t\t\tFHI, Federation of Technology Industries\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"single-item__articles-date\">\n\t\t\t\tNovember 24, 2020\t\t\t<\/div>\n\t\t\t<\/div>\n<\/a>\n\n<a class=\"single-item single-item__articles\" href=\"https:\/\/fhi.nl\/en\/news\/meinberg-announces-new-generation-of-gpsant-antennas\/\" data-id=\"33049\">\n\t<div class=\"single-item__articles-icon\">\n\t\t<svg width=\"35\" height=\"35\" viewbox=\"0 0 35 35\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\">\n<mask id=\"mask0_1182_5532\" style=\"mask-type:luminance\" maskunits=\"userSpaceOnUse\" x=\"0\" y=\"0\" width=\"35\" height=\"35\">\n<path d=\"M0 0H35V35H0V0Z\" fill=\"white\"\/>\n<\/mask>\n<g mask=\"url(#mask0_1182_5532)\">\n<path d=\"M5.12695 9.22852H1.02539V31.9238H10.4299C12.2868 31.9238 14.0678 32.6615 15.3809 33.9746H19.6191C20.9322 32.6615 22.7132 31.9238 24.5701 31.9238H33.9746V11.2793H29.873\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<path d=\"M17.5 9.22852H18.1426C20.0088 7.89544 22.2237 7.17773 24.5615 7.17773H29.873V27.8223H24.5615C22.2237 27.8223 20.0088 28.54 18.1426 29.873H16.8574C14.9912 28.54 12.7763 27.8223 10.4385 27.8223H5.12695V5.12695H9.22852\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<path d=\"M9.22852 1.02539V21.6699C13.759 21.6699 17.5 25.3425 17.5 29.873V9.22852C17.5 4.698 13.759 1.02539 9.22852 1.02539Z\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<\/g>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>Meinberg Announces New Generation of GPSANT Antennas<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Industrial Electronics<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Design Automation &amp; Embedded Systems Event<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Antennas<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Communication technology<\/span>\n\t\t\t\t\t<\/div>\n\t<div class=\"single-item__articles-author-date-wrapper\">\n\t\t\t\t\t<div class=\"single-item__articles-author\">\n\t\t\t\tCN Rood\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"single-item__articles-date\">\n\t\t\t\tJuly 6, 2023\t\t\t<\/div>\n\t\t\t<\/div>\n<\/a>\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/div>","protected":false},"excerpt":{"rendered":"<p>With a combination of smart DNA design tools, rapid assembly methods, Rolling Circle Amplification, and the NEBExpress cell-free protein expression system, you can go from DNA design to purified protein in a single day. This workflow is ideal for high throughput, automation, and testing multiple protein variants simultaneously.<\/p>","protected":false},"featured_media":76026,"template":"","branches":[14],"events":[8],"secretariat":[],"categories":[59,32],"themes_tax":[517],"content_types":[514],"class_list":["post-76025","news","type-news","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Sneller van idee naar eiwit met een effici\u00ebnte DNA en eiwitexpressieworkflow<\/title>\n<meta name=\"description\" content=\"Ontdek hoe je met slimme tools, snelle DNA assemblage en cell free eiwitexpressie binnen \u00e9\u00e9n dag van ontwerp naar gezuiverd eiwit gaat. 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