{"id":84516,"date":"2026-06-25T09:00:00","date_gmt":"2026-06-25T07:00:00","guid":{"rendered":"https:\/\/fhi.nl\/?post_type=news&#038;p=84516"},"modified":"2026-06-23T12:30:12","modified_gmt":"2026-06-23T10:30:12","slug":"proteonano-phosphopeptide-enrichment-kit-voor-phosphoproteomics","status":"publish","type":"news","link":"https:\/\/fhi.nl\/en\/news\/proteonano-phosphopeptide-enrichment-kit-voor-phosphoproteomics\/","title":{"rendered":"Proteonano\u2122 Phosphopeptide Enrichment Kit for phosphoproteomics"},"content":{"rendered":"<header id=\"header\" class=\"header header--high header--branch\">\n\n\t\t\t\t\t\t\t\t\t\t<div class=\"header__background header__background--high\">\n\t\t\t\t\t<img decoding=\"async\" class=\"header__background-image\" src=\"https:\/\/fhi.nl\/app\/uploads\/2026\/06\/phosphopeptide-enrichment-kit-column.avif\" alt=\"\">\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\n\t\n\t<div class=\"container\">\n\t\t<div class=\"header__content\">\n\t\t\t<div class=\"header__first\">\n\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<h1 class=\"header__title\" >\n\t\t\t\t\tProteonano\u2122 Phosphopeptide Enrichment Kit for phosphoproteomics\t\t\t\t<\/h1>\n\n\t\t\t\t<div class=\"header__dots-line\">\n\t\t\t\t\t<svg width=\"431\" height=\"9\" viewbox=\"0 0 431 9\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M430.799 4.192a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.135 1.135 0 1 1-2.27 0 1.135 1.135 0 0 1 2.27 0Zm-27.27 0a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.39 1.39 0 1 1-2.78 0 1.39 1.39 0 0 1 2.78 0Zm-27.78 0a1.645 1.645 0 1 1-3.29 0 1.645 1.645 0 0 1 3.29 0Zm-28.29 0a1.9 1.9 0 1 1-3.799 0 1.9 1.9 0 0 1 3.799 0Zm-28.799 0a2.154 2.154 0 1 1-4.308 0 2.154 2.154 0 0 1 4.308 0Zm-29.308 0a2.41 2.41 0 1 1-4.819 0 2.41 2.41 0 0 1 4.819 0Zm-29.819 0a2.663 2.663 0 1 1-5.326.001 2.663 2.663 0 0 1 5.326-.001Zm-30.327 0a2.919 2.919 0 1 1-5.837 0 2.919 2.919 0 0 1 5.837 0Zm-30.837 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text__content--1-col\">\n\t\t\t<h3>Proteonano\u2122 Phosphopeptide Enrichment Kit for phosphoproteomics<\/h3><p>In proteomics, it is not just about which proteins are present. Sometimes it is also important to know how proteins are regulated.<\/p><p>An important form of regulation is phosphorylation. In this process, a phosphate group is added to a protein. This plays a role in many biological processes, such as cell signaling, growth, and response to stress.<\/p><p>Labs often use phosphoproteomics to investigate these processes. In this process, phosphorylated peptides are analyzed using LC-MS\/MS.<\/p><h2>Why enrichment is important<\/h2><p>Phosphorylated peptides often occur in low amounts. As a result, they can be difficult to measure in complex biological samples.<\/p><p>Therefore, a proper enrichment step is important. This step helps to remove phosphorylated peptides from the sample, making them more visible in the LC-MS\/MS analysis.<\/p><p>For labs working with phosphoproteomics, this step can have a significant impact on the quality of the data.<\/p><h2>What does the Proteonano\u2122 Phosphopeptide Enrichment Kit do?<\/h2><p>The <a href=\"https:\/\/www.mswil.com\/sample-prep\/proteonano-phosphopeptide-enrichment-kitcolumn\/\" data-mce-href=\"https:\/\/www.mswil.com\/sample-prep\/proteonano-phosphopeptide-enrichment-kitcolumn\/\">Proteonano\u2122 Phosphopeptide Enrichment Kit<\/a> has been developed for the enrichment of phosphorylated peptides.<\/p><p>The kit uses TiO\u2082 affinity media. This material binds phosphorylated peptides. Subsequently, these peptides can be washed and removed from the material.<\/p><p>According to the product information from MS Wil, the kit was developed for selective enrichment. This means that the kit is aimed at capturing phosphorylated peptides with as little non-specific binding as possible.<\/p><h2>A simple column workflow<\/h2><p>The column kit uses a ready-to-use tip format. Peptides are loaded onto the TiO\u2082 tips. This is followed by a bind-wash-elute workflow.<\/p><p>This means:<\/p><ul><li><p>binding of phosphorylated peptides<\/p><\/li><li><p>wash to remove harmful substances<\/p><\/li><li><p>elution of the enriched peptides<\/p><\/li><\/ul><p>The enriched peptides can then be analyzed using LC-MS\/MS.<\/p><p>This workflow is intended for labs that want to perform deep and reproducible phosphoproteomics analysis on complex biological samples.<\/p><h2>Data highlights<\/h2><p>The following data highlights are mentioned on the MS Wil product page:<\/p><ul><li><p>23,000+ phosphopeptides<\/p><\/li><li><p>approximately 85% phosphorylation enrichment efficiency<\/p><\/li><li><p>29,000+ phosphosites<\/p><\/li><\/ul><p>These values show that the kit has been developed for deep phosphoproteomics analysis.<\/p><h2>Part of a broader sample prep workflow<\/h2><p>The choice of phosphopeptide enrichment depends on the application. The sample type, the amount of material, and the desired depth also play a role.<\/p><p>Therefore, it is important not to look at just one product. The best choice depends on the entire workflow.<\/p><p><a href=\"https:\/\/www.mswil.com\/\" data-mce-href=\"https:\/\/www.mswil.com\/\">MS Will<\/a> supports European laboratories in finding suitable sample preparation solutions. Within the broader <a href=\"https:\/\/www.mswil.com\/sample-prep\/\" data-mce-href=\"https:\/\/www.mswil.com\/sample-prep\/\">sample prep portfolio<\/a> There are various routes available. Consider protein enrichment, plasma enrichment, digestion, and peptide enrichment.<\/p><h2>Practical support by MS Wil<\/h2><p>For labs that want to perform phosphoproteomics, the <a href=\"https:\/\/www.mswil.com\/sample-prep\/proteonano-phosphopeptide-enrichment-kitcolumn\/\" data-mce-href=\"https:\/\/www.mswil.com\/sample-prep\/proteonano-phosphopeptide-enrichment-kitcolumn\/\">Proteonano\u2122 Phosphopeptide Enrichment Kit<\/a> be a practical route.<\/p><p>The kit assists in the enrichment of phosphorylated peptides for LC-MS\/MS analysis. This can be useful for research into cell signaling, biomarker discovery, and other biological processes in which phosphorylation plays a role.<\/p><p>More information about the Proteonano\u2122 Phosphopeptide Enrichment Kit is available via the MS Wil product page:<br><a href=\"https:\/\/www.mswil.com\/sample-prep\/proteonano-phosphopeptide-enrichment-kitcolumn\/\" data-mce-href=\"https:\/\/www.mswil.com\/sample-prep\/proteonano-phosphopeptide-enrichment-kitcolumn\/\">https:\/\/www.mswil.com\/sample-prep\/proteonano-phosphopeptide-enrichment-kitcolumn\/<\/a><br data-mce-bogus=\"1\"><\/p>\t\t<\/div>\n\t<\/div>\n<\/div>\r\n\t<div class=\"articles bg--offwhite automatic\">\r\n\t\t<div class=\"container\">\r\n\t\t\t<div class=\"articles__header\">\r\n\t\t\t\t\t\t\t\t\t<div class='heading-wrapper'><svg width=\"13\" height=\"13\" viewbox=\"0 0 13 13\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><circle cx=\"6.394\" cy=\"6.5\" r=\"6.394\" fill=\"#000\"\/><\/svg><h2>Related 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stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<path d=\"M17.5 9.22852H18.1426C20.0088 7.89544 22.2237 7.17773 24.5615 7.17773H29.873V27.8223H24.5615C22.2237 27.8223 20.0088 28.54 18.1426 29.873H16.8574C14.9912 28.54 12.7763 27.8223 10.4385 27.8223H5.12695V5.12695H9.22852\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<path d=\"M9.22852 1.02539V21.6699C13.759 21.6699 17.5 25.3425 17.5 29.873V9.22852C17.5 4.698 13.759 1.02539 9.22852 1.02539Z\" stroke=\"#2A5CEE\" stroke-width=\"2\" stroke-miterlimit=\"10\"\/>\n<\/g>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>Automated frying system with integrated Zemic weighing solution<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Laboratory Technology<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button 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fill-rule=\"evenodd\" clip-rule=\"evenodd\" d=\"M71.1889 19.4317C71.1889 18.8156 70.905 18.2339 70.4193 17.8549C69.9336 17.4759 69.3003 17.3419 68.7027 17.4917L39.0059 24.935C38.1162 25.1581 37.4922 25.9577 37.4922 26.875V37.3931H29.2051C28.1005 37.3931 27.2051 38.2885 27.2051 39.3931V75.9996H31.2051V41.3931H47.895V75.9996H51.895V39.3931C51.895 38.2885 50.9996 37.3931 49.895 37.3931H41.4922V28.4356L67.1889 21.9948V75.9998H71.1889V34.3866H77.4785V75.9995H81.4785V32.3866C81.4785 31.282 80.5831 30.3866 79.4785 30.3866H71.1889V19.4317ZM37.5508 51.9436C37.5508 53.0482 38.4462 53.9436 39.5508 53.9436C40.6554 53.9436 41.5508 53.0482 41.5508 51.9436V48.7891C41.5508 47.6845 40.6554 46.7891 39.5508 46.7891C38.4462 46.7891 37.5508 47.6845 37.5508 48.7891V51.9436ZM39.5508 67.01C38.4462 67.01 37.5508 66.1146 37.5508 65.01V61.8555C37.5508 60.7509 38.4462 59.8555 39.5508 59.8555C40.6554 59.8555 41.5508 60.7509 41.5508 61.8555V65.01C41.5508 66.1146 40.6554 67.01 39.5508 67.01ZM56.7871 51.9436C56.7871 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fill=\"#2DD881\"\/>\n<\/svg>\n\t<\/div>\n\t<div class=\"single-item__articles-title\"><div class='heading-wrapper'><h3>W\u00fcrth Elektronik presents new RJ45-10G-LAN transformers<\/h3><\/div><\/div>\n\t<div class=\"single-item__articles-terms\">\n\t\t\n\t\t\n\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Industrial Electronics<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Design Automation &amp; Embedded Systems Event<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">19 inch racks<\/span>\n\t\t\t\t\t\t\t\t<span class=\"button button--outline single-item__articles-term--branche single-item__articles-term\">Active components<\/span>\n\t\t\t\t\t<\/div>\n\t<div class=\"single-item__articles-author-date-wrapper\">\n\t\t\t\t\t<div class=\"single-item__articles-author\">\n\t\t\t\tW\u00fcrth Elektronik NL BV\t\t\t<\/div>\n\t\t\t\t\t\t\t<div class=\"single-item__articles-date\">\n\t\t\t\tMay 26, 2021\t\t\t<\/div>\n\t\t\t<\/div>\n<\/a>\n\t\t\t<\/div>\r\n\t\t<\/div>\r\n\t<\/div>","protected":false},"excerpt":{"rendered":"<p>This article explains how the Proteonano\u2122 Phosphopeptide Enrichment Kit can assist in phosphoproteomics workflows. Phosphorylated peptides often occur in low amounts, making them difficult to measure in complex samples. The kit uses TiO\u2082 affinity media to selectively enrich these peptides, allowing for better analysis using LC-MS\/MS. The article emphasizes that phosphopeptide enrichment is an important sample preparation step for research into cell signaling, biomarker discovery, and other biological processes in which phosphorylation plays a role.<\/p>","protected":false},"featured_media":84517,"template":"","branches":[14],"events":[],"secretariat":[],"categories":[27,427,59,32],"themes_tax":[524,517],"content_types":[514],"class_list":["post-84516","news","type-news","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.8 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Proteonano\u2122 phosphopeptide enrichment | MS Wil<\/title>\n<meta name=\"description\" content=\"Lees hoe de Proteonano\u2122 Phosphopeptide Enrichment Kit helpt om gefosforyleerde peptiden te verrijken voor LC-MS\/MS phosphoproteomics-analyse.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, 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