{"id":78279,"date":"2026-02-03T14:04:43","date_gmt":"2026-02-03T13:04:43","guid":{"rendered":"https:\/\/fhi.nl\/?page_id=78279"},"modified":"2026-03-16T12:44:55","modified_gmt":"2026-03-16T11:44:55","slug":"rethinking-organoid-culture-how-bacterial-proteins-enable-animal-free-2d-and-3d-systems","status":"publish","type":"page","link":"https:\/\/fhi.nl\/en\/rethinking-organoid-culture-how-bacterial-proteins-enable-animal-free-2d-and-3d-systems\/","title":{"rendered":"Rethinking Organoid Culture: How Bacterial Proteins Enable Animal-Free 2D and 3D Systems"},"content":{"rendered":"<header id=\"header\" class=\"header header--medium header--branch\">\n\n\t\n\t\n\t<div class=\"container\">\n\t\t<div class=\"header__content\">\n\t\t\t<div class=\"header__first\">\n\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<div class=\"header__navigation-button\">\n\t\t\t\t\t\t<a class=\"header__navigation-button__arrow\" target=\"_self\" href=\"https:\/\/fhi.nl\/en\/lifescience\/programma\/\">\n\t\t\t\t\t\t\t<svg width=\"56\" height=\"33\" viewbox=\"0 0 56 33\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><rect x=\"56\" y=\"33\" width=\"56\" height=\"33\" rx=\"16.5\" transform=\"rotate(180 56 33)\" fill=\"#ffda56\"\/><path d=\"M22.262 22.456 16.294 16.5l5.968-5.956M39.706 16.5H16.293\" stroke=\"#fff\" stroke-width=\"2\" stroke-linecap=\"round\"\/><\/svg> To program\t\t\t\t\t\t<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<h1 class=\"header__title\" >\n\t\t\t\t\tRethinking Organoid Culture: How Bacterial Proteins Enable Animal-Free 2D and 3D Systems\t\t\t\t<\/h1>\n\n\t\t\t\t<div class=\"header__dots-line\">\n\t\t\t\t\t<svg width=\"431\" height=\"9\" viewbox=\"0 0 431 9\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M430.799 4.192a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.135 1.135 0 1 1-2.27 0 1.135 1.135 0 0 1 2.27 0Zm-27.27 0a1.136 1.136 0 1 1-2.272-.001 1.136 1.136 0 0 1 2.272 0Zm-27.272 0a1.39 1.39 0 1 1-2.78 0 1.39 1.39 0 0 1 2.78 0Zm-27.78 0a1.645 1.645 0 1 1-3.29 0 1.645 1.645 0 0 1 3.29 0Zm-28.29 0a1.9 1.9 0 1 1-3.799 0 1.9 1.9 0 0 1 3.799 0Zm-28.799 0a2.154 2.154 0 1 1-4.308 0 2.154 2.154 0 0 1 4.308 0Zm-29.308 0a2.41 2.41 0 1 1-4.819 0 2.41 2.41 0 0 1 4.819 0Zm-29.819 0a2.663 2.663 0 1 1-5.326.001 2.663 2.663 0 0 1 5.326-.001Zm-30.327 0a2.919 2.919 0 1 1-5.837 0 2.919 2.919 0 0 1 5.837 0Zm-30.837 0a3.173 3.173 0 1 1-6.345.001 3.173 3.173 0 0 1 6.345 0Zm-31.346 0a3.428 3.428 0 1 1-6.856 0 3.428 3.428 0 0 1 6.856 0Zm-31.856 0a3.683 3.683 0 1 1-7.365 0 3.683 3.683 0 0 1 7.365 0Zm-32.365 0a3.937 3.937 0 1 1-7.875 0 3.937 3.937 0 0 1 7.875 0Zm-32.874 0a4.192 4.192 0 1 1-8.384 0 4.192 4.192 0 0 1 8.384 0Z\" fill=\"#FFF960\"\/><\/svg>\t\t\t\t<\/div>\n\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"header__description\">\n\t\t\t\t\t\t\t<p>Joost Wijnakker<\/p>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\n\t\t\t<\/div>\n\n\t\t\t\t\t\t\t<div class=\"header__second\">\n\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"header__branch-logos\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/fhi.nl\/app\/uploads\/2024\/02\/Laboratorium-technologie.svg\" class=\"header__branch-logo\" alt=\"\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t\t\n\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/header>\n\n\t<div class=\"header__meta\">\n\t<div class=\"container\">\n\t\t<div class=\"header__meta__category\">\n\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Branch<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_branches_kennishub=laboratorium-technologie\" class=\"header__meta__detail--branch\">\n\t\t\t\t\t\t\t\tLaboratory Technology\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t\t\t\t\t\t<div class=\"header__meta__detail\">\n\t\t\t\t\t<div>Subject<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/kennishub\/?_onderwerp_kennishub=laboratorium-automatisering\" class=\"header__meta__detail--categorie\">\n\t\t\t\t\t\t\t\tLaboratory automation\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/div>\n\n\n\n<div class=\"text bg--offwhite\">\n\t<div class=\"container\">\n\t\t\t\t\t<div class='heading-wrapper'><svg width=\"13\" height=\"13\" viewbox=\"0 0 13 13\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><circle cx=\"6.394\" cy=\"6.5\" r=\"6.394\" fill=\"#000\"\/><\/svg><h2>09.00 \u2013 09.30<\/h2><\/div>\t\t\t\t\t\t<div class=\"text__content text__content--1-col\">\n\t\t\t<p>Organoids have become indispensable tools in fundamental research and personalized medicine\u2014but many labs still rely on animal-derived matrices (Matrigel) that are expensive, variable, and difficult to standardize. What if one simple recombinant protein could offer a smarter alternative? Recent advances show that the bacterial protein invasion can be repurposed to support robust organoid growth in fully defined, animal-free systems. From 2D monolayers suited for high-throughput screening to long-term 3D cultures using a synthetic PIC gel, these approaches combine practicality with physiological relevance. Together, they pave the way toward standardized organoid culture, reduced reliance on animal-derived materials, and open new possibilities for innovative applications of organoid technology.<\/p>\n<p>\u2013 Joost Wijnakker \u2013<\/p>\n<p>&nbsp;<\/p>\n\t\t<\/div>\n\t<\/div>\n<\/div>\n\n\n<div class=\"image bg--white\">\n\t<div class=\"container\">\t\t\t<div class='heading-wrapper'><svg width=\"13\" height=\"13\" viewbox=\"0 0 13 13\" fill=\"none\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><circle cx=\"6.394\" cy=\"6.5\" r=\"6.394\" fill=\"#000\"\/><\/svg><h2>Featured article<\/h2><\/div>\t\t<div class=\"image__wrapper\">\n\n\t\t\n\t\t\t\t\t<div class=\"image__content\">\n\t\t\t\t\t\t\t\t\t<div class=\"image__titles\">\n\t\t\t\t\t\t<div class='heading-wrapper'><h2 class='image__title'>Animal-free breakthrough in organoid cultivation<\/h2><\/div>\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"image__subtitle\">His research, conducted within the Clevers group under the supervision of Hans Clevers and Patricia Dankers, focused on breaking a persistent reliance in the organoid field: the use of Matrigel, an animal product derived from mouse tumors.<\/span>\n\t\t\t\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/fhi.nl\/en\/nieuws\/diervrije-doorbraak-in-de-organoidenkweek\/\" target=\"_blank\" class=\"image__button button button--secondary\">Read more<\/a>\n\t\t\t\t\t\t\t<\/div>\n\t\t\n\t\t\t\t\t<div class=\"image__overlay\"><\/div>\n\t\t\n\t\t<img decoding=\"async\" width=\"1728\" height=\"1080\" src=\"https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot-1728x1080.png\" class=\"attachment-image_block size-image_block\" alt=\"Life science\" style=\"object-position: center center;\" srcset=\"https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot-1728x1080.png 1728w, https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot-300x188.png 300w, https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot-1024x640.png 1024w, https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot-768x480.png 768w, https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot-1536x960.png 1536w, https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot-2048x1280.png 2048w, https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot-18x12.png 18w, https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot-576x360.png 576w, https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot-453x283.png 453w, https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot-200x125.png 200w, https:\/\/fhi.nl\/app\/uploads\/2024\/05\/Afbeelding-AMR-groot.png 1920w\" sizes=\"(max-width: 1728px) 100vw, 1728px\" \/>\t<\/div>\n\t<\/div><\/div>","protected":false},"excerpt":{"rendered":"","protected":false},"author":44,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"branches":[14],"events":[369],"secretariat":[],"categories":[30],"themes_tax":[538],"class_list":["post-78279","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.8 - 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