Holographic Microscopy. This unique technology brings real-time label free monitoring to suspension cell bioreactors. In Digital Holography, the scattered light beam from an illuminated object interferes with a reference beam on a CCD camera allowing for a 3D numerical reconstruction of the object.
Digital Holography can construct intensity images, quantitative phase images and 3D images covering the shape and density of an object in a volume. The phase image is equivalent to the optical thickness of the sample. Images of cells captured in a hologram can be refocused after acquisition.
The iLine Fast measures the specific holographic signature of each cell within the field of view and computes a cell count based on a sequence of holograms. The platform shows cell density and cell viability per measurement sequence.
The iLine Fast is designed specifically for use in combination with bioreactors. The technology can track a full cycle and can count cell viability from very low to high concentrations.
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Mathuis Philip, Ovizio – in collaboration with Applikon Biotechnology |