The new Vanquish Duo UHPLC system is a novel technology that focuses on productivity across all markets. It offers three workflows and two independent flow paths in one integrated solution. These workflows are consistent or true Dual LC using two independent flow paths, Tandem LC or LC-MS and Inverse Gradients.
Industry is using dual LC to increase throughput by running samples in parallel or gain additional characterization applications in parallel. For example, monitoring antibody charge variants by ion-exchange chromatography on one channel, while at the same time performing aggregate analysis of the same sample by size exclusion chromatography.
Tandem LC or LC-MS allows all chromatographers to improve their performance. This is accomplished by the system’s dual gradient pump allowing the analytical gradient to be run on one column while the second column is being reconditioned in parallel. The next analytical gradient is then run on the second column while the first column is being reconditioned, thus saving the time of column reconditioning experienced on a single system.
Inverse Gradient technology is especially useful for those who are regular users of universal detectors such as charged aerosol detection (CAD). CAD is prized as it allows the detection of analytes that lack chromophore or ionizing and in addition allows the relative quantification of analytes without the need for reference standards. However, one limitation or universal detectors is that changing eluent composition can lead to a non-uniform response which impedes quantitation. The Inverse Gradient on the Vanquish Duo alleviates this is the second pump to produce an inverse gradient to the analytical gradient of the first pump. The two channels are then combined before a consistent eluent composition entering the detector. This ensures a uniform response for quantitation.
In total, the Vanquish Duo UHPLC system offers lower costs per sample, increased throughput, maximum return on investment (ROI) or instrument purchases and efficient bench-space utilization, all leading to higher productivity. The Vanquish Duo has the potential to be the UHPLC system of choice for liquid chromatography separations due to its unique productivity benefits and other low throughput separation technologies such as electrophoresis.
For more information on the Vanquish Duo, please visit www.thermofisher.com/VanquishDuo
Video link: https://www.youtube.com/watch?v=Xh_4mN7M3bo&t=53s
