Automate the determination of 3-MCPD and glycidol in edible oils

3-MCPD, Glycidol and their fatty acid esters are contaminants, which are formed during the production of edible oils and fats. Because these contaminants are possibly carcinogenic to humans, rules and regulations have been established for the daily intake values and the maximum levels in edible oils. Various analytical methods are available for the determination such as ISO 18363-1, AOCS Cd 29c-13 and DGF C-VI 18 (10).  

A GERSTEL case study describes how the determination of 3-MCPD and Glycidol in edible oils can be fully automated according to ISO 18363-1. For this study, automated sample preparation was carried out on a GERSTEL MultiPurpose Sampler in DualHead version equipped with the Quickmix: an important module for mixing during the liquid / liquid extraction steps. For the required evaporation of the samples during derivatization, the GERSTEL mVap was used, which automatically removes the excess derivatization reagent and thus keeps the analysis stable. The sample is then injected via a cooled injection system CIS 4 PTV. An Agilent 7890 Gas chromatograph coupled to an Agilent 5977 MSD was used for separation and detection. The study shows that method ISO 18363-1 can be automated using the GERSTEL MPS and that the results obtained correlate well with reference data. This method is comparable to two other commonly used methods: AOCS Cd 29c-13 and DGS C-VI 18 (10). The excellent relative standard deviations for the complete process, including GC/MS analysis, argue for automation of this determination. 

Visit the Da Vinci Laboratory Solutions stand during LabAnalyse for more information about this application or download the application note via the Da Vinci website.