Door: Bio-Rad Laboratories The Western Blot Doctor is a self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems. Comprehensive solutions and suggestions are provided to help solve your particular western blotting challenges.with the Western Blot Doctor™
Tips, Techniques, and Technologies from the Western Blotting Experts at Bio‑Rad Laboratories: Get Your Free Guide
The Western Blot Doctor is a self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems. Comprehensive solutions and suggestions are provided to help solve your particular western blotting challenges.
To start troubleshooting your western blotting problems, choose the type of problem you are experiencing.
I am having trouble with:
- Inconsistent control protein levels across samples
- Ghost protein bands
- Swirls or missing bands; bands appear diffuse on blot
- Blurry bands
- Bands are curved (smiling) not straight
- Broad or misshapen bands
- White (negative) bands on film using ECL method
- No bands
- Band(s) at lower MW than expected
- Band(s) at slightly higher MW than expected, and may be blurry
- Band(s) at significantly higher MW than expected
- Multiple bands at various MWs
- White spots or regions on membrane
- High background on blot
- Blotchy or patchy background
- Uneven spots on blot or speckled background
- Shadow of tank blotter gel cassette on blot
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