Proteonano™ Phosphopeptide Enrichment Kit for phosphoproteomics
In proteomics, it is not just about which proteins are present. Sometimes it is also important to know how proteins are regulated.
An important form of regulation is phosphorylation. In this process, a phosphate group is added to a protein. This plays a role in many biological processes, such as cell signaling, growth, and response to stress.
Labs often use phosphoproteomics to investigate these processes. In this process, phosphorylated peptides are analyzed using LC-MS/MS.
Why enrichment is important
Phosphorylated peptides often occur in low amounts. As a result, they can be difficult to measure in complex biological samples.
Therefore, a proper enrichment step is important. This step helps to remove phosphorylated peptides from the sample, making them more visible in the LC-MS/MS analysis.
For labs working with phosphoproteomics, this step can have a significant impact on the quality of the data.
What does the Proteonano™ Phosphopeptide Enrichment Kit do?
The Proteonano™ Phosphopeptide Enrichment Kit has been developed for the enrichment of phosphorylated peptides.
The kit uses TiO₂ affinity media. This material binds phosphorylated peptides. Subsequently, these peptides can be washed and removed from the material.
According to the product information from MS Wil, the kit was developed for selective enrichment. This means that the kit is aimed at capturing phosphorylated peptides with as little non-specific binding as possible.
A simple column workflow
The column kit uses a ready-to-use tip format. Peptides are loaded onto the TiO₂ tips. This is followed by a bind-wash-elute workflow.
This means:
binding of phosphorylated peptides
wash to remove harmful substances
elution of the enriched peptides
The enriched peptides can then be analyzed using LC-MS/MS.
This workflow is intended for labs that want to perform deep and reproducible phosphoproteomics analysis on complex biological samples.
Data highlights
The following data highlights are mentioned on the MS Wil product page:
23,000+ phosphopeptides
approximately 85% phosphorylation enrichment efficiency
29,000+ phosphosites
These values show that the kit has been developed for deep phosphoproteomics analysis.
Part of a broader sample prep workflow
The choice of phosphopeptide enrichment depends on the application. The sample type, the amount of material, and the desired depth also play a role.
Therefore, it is important not to look at just one product. The best choice depends on the entire workflow.
MS Will supports European laboratories in finding suitable sample preparation solutions. Within the broader sample prep portfolio There are various routes available. Consider protein enrichment, plasma enrichment, digestion, and peptide enrichment.
Practical support by MS Wil
For labs that want to perform phosphoproteomics, the Proteonano™ Phosphopeptide Enrichment Kit be a practical route.
The kit assists in the enrichment of phosphorylated peptides for LC-MS/MS analysis. This can be useful for research into cell signaling, biomarker discovery, and other biological processes in which phosphorylation plays a role.
More information about the Proteonano™ Phosphopeptide Enrichment Kit is available via the MS Wil product page:
https://www.mswil.com/sample-prep/proteonano-phosphopeptide-enrichment-kitcolumn/