DeNovix DS-11

DS-11 Series

Award Winning Performance

DeNovix takes spectrophotometry to a whole new level of performance with the DS-11 Series. This easy-to-use, compact instrument delivers full spectrum UV-Vis analysis and fluorescence capability making it ideal for rapid nucleic acid and protein quantification. Our customers agree and have made the DeNovix DS-11 Series the twice winner of the Reviewers’ Choice Product of the Year and the first life science instrument to be awarded the Platinum Seal of Quality by SelectScience based on scientist reviews for outstanding ease-of-use, performance, service and technical support.

Best in Class 1 uL Sensitivity and Highest Absorbance Capability

DS-11 Series Spectrophotometers set the industry standard for the lower detection limits of 0.5 -1.0 uL UV-Vis sample analysis. Detect to 0.75 ng/uL dsDNA, 0.60 ng/uL RNA or 0.04 mg/mL BSA. The DS-11 can measure samples with absorbance values as high as 750 absorbance units (at a 1 cm equivalent path length). This translates into 1125 mg/ml of BSA protein or 37500 ng/uL of dsDNA. This ultra high absorbance capability is achieved by using a remarkably small pathlength enabled by our SmartPath® Technology.

Easy to Learn. Fast to Use.

The DS-11 Series instruments feature the most powerful and intuitive software of its type. EasyApps® software is designed by life scientists and combines with an intuitive touchscreen interface to ensure error-free operation and sample to data in under 4 seconds. Every instrument is ready to go right out of the box – no PC set-up or software installation required.

INTEGRATED ABSORBANCE AND FLUORESCENCE FOR UNMATCHED FLEXIBILITY

The Only All-In-One Microvolume and Cuvette Absorbance with Fluorescence Design

With the DS-11 Series you can equip your lab for today’s quantification requirements and for future assay challenges. Combine the ease of use, wide dynamic range and contamination information of UV-Vis with the enhanced sensitivity and specific quantification of fluorescence for total sample quality control.

Absorbance and Fluorescence quantification are unique but complementary methods for assessing concentration and quality of biological samples. The multi-award winning DS-11 Series of 1 µL Spectrophotometers and Fluorometers provides the most sensitive and widest dynamic range for UV-Vis absorbance and fluorescence capability in one combined instrument.

Sensitivity
Extend the lower detection limit for low concentration samples. Quantify down to 0.5 pg/µL dsDNA

Specificity
Selectively quantify your sample in the presence of contaminants using DeNovix Fluorescence Quantification Assays

Total Sample QC

Combine Absorbance and Fluorescence measurements for specific quantification and reliable contamination detection.

21 CFR Part 11 Compliance Ready

EasyApps Secure provides an optional suite of software controls that allow regulated GxP facilities to easily add DS-11 Series instruments to their compliant workflows (view the compliance statement). The software is fully integrated within the on-board operating system and includes a range of features essential to ensuring compliance, including:

Password protected system access
Integrated electronic signature controls
Comprehensive user account management
Secure audit trail recording
Advanced data handling and export tools

Software Features

Nucleic Acid Quantification

Separate preconfigured apps for optimized workflow
Sample contaminant alerts
Preconfigured and user defined factors for ssDNA ensures appropriate concentration calculations
260/280 and 260/230 ratios displayed
11 Preconfigured dyes and fluorophores for Microarray
Option to add an unlimited number of new dyes for quick recall in Microarray

Protein Quantification

Preconfigured and user defined protein types for A280 and Labeled Proteins
Preconfigured Peptide app for easy peptide analysis at A205/215
Quick addition of new user defined protein types for improved workflow
Ultra short pathlength enables high absorbance capability: up to 750 mg/mL BSA
11 Preconfigured dyes and fluorophores for Labeled Proteins
Option to add an unlimited number of new dyes for quick recall in Labeled Proteins
BCA, Bradford, Lowry and 660 nm colorimetric assays optimized for ease of use
Quick save and recall of standard curves available

UV-Vis and Custom Apps

Simple but powerful apps enable measurement of more than just nucleic acids and proteins
Full spectrum analysis (190 – 840 nm)
Broad absorbance range for highly concentrated samples (up to 750 A, 1 cm equivalent)
Add new user-defined standard curve methods
Add custom formulas to eliminate downstream calculations
Add kinetics methods (DS-11+/DS-11FX+ models)

Fluorescence Quantification

Four user-selectable LED light sources – compatible with all commonly used quantification assays e.g. DeNovix, Qubit®, Quant-itTM and Qunatifluor®
Easy configuration of additional assays
Single or multi-replicate standard curve options
User control of line fit, trend line analysis, subtraction of blank
User control of assay and reported sample concentration units
Save and re-use curves and lists of standard concentrations

Custom Fluorescence Apps

Add new standard curve based fluorescent assays (nucleic assay, protein or other)
Basic Fluorometer app returns RFU values for a wide range of fluorophores

Kinetics and OD600

DS-11+ model enables cuvette-based kinetics studies
Full spectrum analysis (190 – 840 nm), with dual or single wavelength monitoring
Built-in heater with temperature control between 37° and 45° C.
Dual graphs enable absorbance vs time or absorbance vs. wavelength plots
All kinetic data saved
Estimated cell number calculator included in OD 600 app

User Accounts and Data Handling

Password protected accounts available
All data automatically saved & searchable by account, sample name & type, application and date
Configurable reports easily accommodate LIMS and user preferred formats
Print microfuge tube labels using a DeNovix supported printer
Print reports to a networked printer or DeNovix supported label printer
Export full data sets via email; save to a network folder or USB thumb drive.

Exporting

Simply and securely connect the DS-11 to your network by Wifi or Ethernet to enable data exporting by email, or print reports to network or label printers. Each measurement is automatically saved to a large onboard memory enabling storage and retrieval of millions of samples. For labs requiring external data storage, network folder locations can be defined for each user account to automatically export and archive results.

Multiple Model and Color Choices

The DS-11 Series is available in 4 models with different combinations of microvolume absorbance (M), cuvette absorbance (C) and Fluorescence (F):

DS-11 FX+ (M/C/F)
DS-11 FX (M/F)
DS-11+ (M/C)
DS-11 (M)

All models are available in multiple colors (red, white, blue and silver)

Specifications

Spectrophotometer Microvolume Mode (DS-11, DS-11+, DS-11 FX, DS-11 FX+)

Minimum Sample Size	0.5 µL
Pathlength	0.5 mm (auto ranging to 0.02 mm)
Light Source	Pulsed Xenon flash lamp
Detector Type	2048 element CCD
Wavelength Range	190-840 nm
Wavelength Accuracy	0.5 nm
Wavelength Precision	0.14 nm
Spectral Resolution	1.5 nm (FWHM at Hg 253.7 nm)
Absorbance Precision	0.015 AU (1 cm) or 1%, whichever is greater
Absorbance Accuracy	1.5% at 0.75 AU at 260 nm
Absorbance Range	0.015 – 750 (1 cm equivalent)
Detection limit	0.04 mg/mL BSA; 0.75 ng/µL dsDNA
Maximum Concentration	1125 mg/mL BSA; 37500 ng/µL dsDNA
Lower Sample Surface Material of Construction	303 stainless steel and sapphire window
Upper Sample Surface Material of Construction	303 stainless steel and quartz fiber

Spectrophotometer Cuvette Mode (DS-11+, DS-11 FX+)

Beam height	8.5 mm
Heating	37-45 °C +/- 0.5 °C
Pathlength	10, 5, 2, 1, 0.5, 0.2, 0.125 mm
Cuvette Dimensions	12.5 mm x 12.5 mm x 45 mm (LxWxH)
Absorbance Range	0.0008-1.5 AU (1 cm)
Detection Limit	0.002 mg/mL BSA; 0.04 ng/µL dsDNA (1 cm)
Maximum Concentration	75 ng/µL dsDNA (1 cm)

Fluorometer Mode (DS-11 FX, DS-11 FX+ and FX Module)

Light Sources	UV LED (~375 nm), Blue LED (~470 nm), Green LED (~525 nm), Red LED (~635 nm)
Excitation Filters	UV: 361-389 nm, Blue: 442-497 nm, Green: 490-558 nm, Red: 613-662 nm
Emission Filters	435-485 nm , 514-567 nm, 565-650 nm, 665-740 nm
Detectors	Photodiode, detection range 300-1000 nm
Tube Type	0.5 mL Real Time thin-wall PCR tube (polypropylene)

Onboard Controller (No PC required)

Operating System	Custom Linux® OS
CPU	Quad-core Cortex-A72
Display	7” high definition color display
User Input	Touch screen with swipe and pinch motion; glove compatible
Internal Storage	32GB flash drive
Connectivity	3 USB ports for printer, barcode reader and USB drive
Networking	Ethernet and Wi-Fi

General

Colors	Arctic White, Brazilian Blue, Fire Red, Tungsten Silver
Measurement Time for Spectrophotometry	2 seconds
Measurement Time for Fluorescence	2 seconds
Footprint	20 cm X 33 cm
Weight	2 kg
Operating Voltage	12 VDC
Operating Power Consumption	10 W; max 30 W
Approvals	CE, UL/CSA, FCC and Japan CAB
Manufacture Location	USA

FX Module: Upgrade to Fluorescence

Instantly add the power of fluorescence detection to any DeNovix DS-11, DS-11+ or DS-C Spectrophotometer. The FX Module uses the same fluorescence detection as the DS-11 FX, DS-11 FX+ and QFX. Simply plug in this USB powered accessory and DeNovix fluorometric EasyApps® are immediately enabled. With four user-selectable LEDs, the easy-to-use apps are compatible with a wide range of commercially available assays including those designed for Qubit® and Quantus™ platforms.

Absorbance and Fluorescence Quantification Infographic

Selecting the appropriate quantification methods for biological samples is an important consideration in any quality control process. Download our infographic featuring comparisons of the key features of absorbance and fluorescence quantification and information on when each method should be applied or the results combined.